NP-Fluorescein-CGG MF: C8H7N2O4-Fluorescein-CGG
Fluorescein conjugated to NP-CGG via amide bonds to lysine.

NP-Fluorescein-CGG

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Alternate Names: 4-Hydroxy-3-nitrophenylacetyl-Chicken Gamma Globulin-Fluorescein
Application: NP-Fluorescein-CGG is Fluorescein conjugated to NP-CGG via amide bonds to lysine
Molecular Formula: C8H7N2O4-Fluorescein-CGG
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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NP-Fluorescein-CGG (4-Hydroxy-3-nitrophenylacetyl-Chicken Gamma Globulin-Fluorescein) is a fluorescein conjugated to NP-CGG via amide bonds to lysine.

Absorption Maximum (Lambda Max): 430 nm
Extinction Coefficient at Lambda max: 4230/M/cm


References

1. Shimizu, Takeyuki., et al., 2004. Affinity maturation of secreted IgM pentamers on B cells. International immunology. 16(5): 675-84. PMID: 15096487

Usage :
This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1 mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant.

Store solution in aliquots at -20°C. Protect from light.

Conjugation is determined by measuring the absorbance of NP at 495 nm, using 4.23 x 10E+03 as the extinction coefficient for NP, and is given as a molar ratio of NP to CGG assuming the molecular weight of CGG is 150,000.
Conjugation of Fluorescein is determined by measuring the absorbance at 495 nm using 8.3 x 10E+04 as the extinction coefficient of Fluorescein and is given as a molar ratio of fluorescein to NP-CGG
Appearance :
Powder
Physical State :
Solid
Storage :
Desiccate at 4° C
Fluoresence :
λex 430 nm, λem 423 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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