NP-CGG (Chicken Gamma Globulin), Ratio 30-39 An NP hapten conjugated to CGG via amide bonds to lysine.

NP-CGG (Chicken Gamma Globulin), Ratio 30-39

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Alternate Names: 4-Hydroxy-3-nitrophenylacetyl-CGG
Application: NP-CGG, Ratio 30-39 is an NP hapten conjugated to CGG via amide bonds to lysine
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

NP-CGG, Ratio 30-39, is a 4-Hydroxy-3-nitrophenylacetyl hapten conjugated to CGG via amide bonds to lysine.

Absorption Maximum (Lambda Max): 430 nm
Extinction Coefficient at Lambda max: 4230/M/cm


References

1. Hawkins, R E., et al., 1992. Cell selection strategies for making antibodies from variable gene libraries: trapping the memory pool. European journal of immunology. 22(3): 867-70. PMID: 1547829
2. Vora, K A., et al., 1997. Amplified follicular immune complex deposition in mice lacking the Fc receptor gamma-chain does not alter maturation of the B cell response. Journal of immunology (Baltimore, Md. : 1950). 159(5): 2116-24. PMID: 9278297
3. Rada, C., et al., 1998. Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting. Immunity. 9(1): 135-41. PMID: 9697843

Usage :
Conjugation is determined at pH 8.5 by measuring NP absorbance at 430nm, using the extinction coefficient of NP= 4.23 x 10E+03. The conjugation ratio is given as a molar ratio of NP to CGG assuming the molecular weight of CGG is 150,000.

Store solution in aliquots at -20°C. Protect from light.

This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant.
Appearance :
Powder
Physical State :
Solid
Storage :
Desiccate at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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