normal mouse IgG 2a normal unconjugated immunoglobulin for use as a negative control

normal mouse IgG2a: sc-3878

normal mouse IgG2a is rated 5.0 out of 5 by 2.
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Datasheets

    • sufficient for 100 tests
    • non-conjugated
    • affinity purified immunoglobulin
    • for use as negative controls for flow cytometry
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Could you confirm whether this antibody could be used as an isotype control for the IP experiment? Any publications used this antibody for IP? Thanks!

Asked by: iconsdkxq7
Thank you for your question. Normal mouse IgG2a: sc-3878 is suitable as an isotype control for any experiment using an unconjugated primary IgG2a of murine origin. IP use of this antibody is reprted in several publications, including PMID 29912633, PMID 27472061, and PMID 19649319.
Answered by: Tech Support Europe
Date published: 2020-12-28

Could you please confirm if this is an isotype control suitable for immunofluorescence and the concentration?

Asked by: Livia
Thank you for your question. Yes, this normal mouse IgG is suitable as a negative control for immunofluorescence. It is provided as 50µg/ml.
Answered by: Tech Service
Date published: 2021-05-20

What is the concentration of this antibody? Is it the same as any other antibodies provided by Santa Cruz Biotechnology?

Asked by: GosiaD
Thank you for your question. This normal mouse IgG2a, sc-3878, is provided at a concentration of 50µg/ml.
Answered by: Tech Service
Date published: 2020-08-19

I want to use this antibody for IP isotope control. What's the concentration ?

Asked by: X123
Thank you for your question. This isotype control, normal mouse IgG2a: sc-3878, is titrated for use with our flow cytometry protocol, and that is why it is recommended for 100 tests. However, it can be used as an isotype control in other applications (including IP) and the concentration is 100 µg in 2 mL.
Answered by: Technical Support
Date published: 2020-08-19
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Rated 5 out of 5 by from good control antibody good control antibody for FC analysis, recommend use.
Date published: 2017-03-18
Rated 5 out of 5 by from Never encounter problems using this product Never encounter problems using this product.
Date published: 2015-03-27
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