Anti-NKCC2 Antibody (4H4) recommended for detection of NKCC2 of human origin by WB, IP and ELISA

Anti-NKCC2 Antibody (4H4): sc-293222

Anti-NKCC2 Antibody (4H4) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-NKCC2 Antibody (4H4) is a mouse monoclonal IgG2a (kappa light chain) NKCC2 antibody provided at 100 µg/ml
    • raised against amino acids 80-172 of NKCC2 of human origin
    • recommended for detection of NKCC2 of human origin by WB, IP and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NKCC2 (4H4): sc-293222.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for NKCC2 Antibody (4H4) for WB applications. This reagent is now offered in a bundle with NKCC2 Antibody (4H4) (see ordering information below).

For Western Blot, is it recommended to use denatured or non-denatured conditions with NKCC2 (4H4): sc-293222 antibody?

Asked by: cjMara
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from non ha funzionato purtroppo l'anticorpo non ha funzionato sui preparati di rene di teleosteo. Anche quando ho eseguito un controllo positivo su rene di mammifero il risultato è stato deludente.
Date published: 2017-07-10
Rated 5 out of 5 by from Great antibody The NKCC2 antibody worked really well at 1:50 and 1:100 dilutions. Pictures below are 1:50 dilutions.
Date published: 2017-04-13
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