Anti-NKCC2 Antibody (4H4) is a mouse monoclonal IgG2a (kappa light chain) NKCC2 antibody provided at 100 µg/ml
raised against amino acids 80-172 of NKCC2 of human origin
recommended for detection of NKCC2 of human origin by WB, IP and ELISA
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NKCC2 (4H4): sc-293222.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for NKCC2 Antibody (4H4) for WB applications. This reagent is now offered in a bundle with NKCC2 Antibody (4H4) (see ordering information below).
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possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
NKCC2 Antibody (4H4) is a high quality monoclonal NKCC2 antibody (also designated NKCC2 antibody) suitable for the detection of the NKCC2 protein of human origin. NKCC2 Antibody (4H4) is available as the non-conjugated anti-NKCC2 antibody. Na-K-Cl cotransporters (NKCC) are channel proteins that aid in the transcellular movement of chloride across both secretory and absorptive epithelia. NKCC1 is expressed in muscle cells, neurons, and red blood cells. In the basolateral membrane of secretory epithelia, NKCC1 mediates active chloride secretion. The gene encoding human NKCC1 maps to chromosome 5q23.3. In mice, disruption of the NKCC1 gene leads to deafness and impaired balance. NKCC2 is specifically expressed in the kidney where it mediates active reabsorption of sodium chloride in the thick ascending limb of the loop of Henle. NKCC2 is sensitive to the clinically important diuretics furosemide and bumetanide. The gene encoding human NKCC2 maps to chromosome 15q15-q21 and mutations in this gene lead to Bartter′s syndrome, an inherited hypokalaemic alkalosis. NCCT is a thiazide-sensitive Na-Cl cotransporter that is primarily expressed in the distal convoluted tubule of the kidney where it accounts for a significant fraction of net renal sodium reabsorption. The gene for human NCCT map to chromosome 16q13. Mutations in the gene encoding NCCT cause Gitelman′s syndrome, a subset of Bartter′s syndrome.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
For Western Blot, is it recommended to use denatured or non-denatured conditions with NKCC2 (4H4): sc-293222 antibody?
Asked by: cjMara
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Rated 1 out of
stella 2017 from
non ha funzionatopurtroppo l'anticorpo non ha funzionato sui preparati di rene di teleosteo. Anche quando ho eseguito un controllo positivo su rene di mammifero il risultato è stato deludente.
Date published: 2017-07-10
Rated 5 out of
Great antibodyThe NKCC2 antibody worked really well at 1:50 and 1:100 dilutions. Pictures below are 1:50 dilutions.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn