NGFR p75 Antibody (ME20.4)recommended for detection of NGFR p75 of mouse, rat and human origin by WB, IP, IF and IHC(P)

NGFR p75 Antibody (ME20.4): sc-13577

NGFR p75 Antibody (ME20.4) is rated 4.3 out of 5 by 8.
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Datasheets

    • NGFR p75 Antibody (ME20.4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against WM245 melanoma cells of human origin
    • NGFR p75 Antibody (ME20.4) is recommended for detection of NGFR p75 of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including and bovine, porcine, feline and canine
    • NGFR p75 Antibody (ME20.4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NGFR p75 (ME20.4): sc-13577.
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What is the recommended fixation method for immunofluorescence staining with NGFR p75 (ME20.4): sc-13577 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from One lane The antibody anti-NGFR p75 (ME20.4; sc-13577) was tested by Western blotting analysis. The samples loaded (30 μg total of proteins) derive from human cancer cells known to be positive (ctrl +) or negative (ctrl -) for the p75 expression. As shown in the western blot, the antibody recognizes only a band corresponding to 75 KDa. Despite the high amount of proteins loaded the bands observed are very faint. Probably the primary antibody must be used more concentrated (e.g 1:200) Western blot conditions: Blocking 5% Milk (1h- RT); Ab I 1:500 in tTBS overnight (+4°C) ; Ab II 1:2000 anti-mouse in 3% Milk (1h-RT)
Date published: 2019-05-31
Rated 5 out of 5 by from Methanol fixed IF is Great IF staining of methanol fixed cells shows excellent membrane localization.
Date published: 2017-01-20
Rated 5 out of 5 by from Published IHC data for use in thymic cells Published IHC data for use in thymic cells -SCBT Publication Review
Date published: 2015-05-05
Rated 4 out of 5 by from Published data in IHC using human oral squamous Published data in IHC using human oral squamous cell carcinoma samples -SCBT Publication Review
Date published: 2015-04-27
Rated 4 out of 5 by from Great immunofluorescence membrane staining Great immunofluorescence membrane staining in methanol-fixed Hs 294T cells. -SCBT QC
Date published: 2015-04-05
Rated 4 out of 5 by from Very favorable WB band achieved using SK Very favorable WB band achieved using SK-N-MC whole cell lysate -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Published data in RPE cells using this antibody Published data in RPE cells using this antibody for Western blot analysis -SCBT Publication Review
Date published: 2015-01-14
Rated 4 out of 5 by from Nice publishable data on Western blot on Nice publishable data on Western blot on human brain tissue. -SCBT Publication Review
Date published: 2015-01-07
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