NGFR p75 Antibody (MC-192)recommended for detection of NGFR p75 of mouse and rat origin by WB, IP, IF and FCM

NGFR p75 Antibody (MC-192): sc-53631

NGFR p75 Antibody (MC-192) is rated 4.5 out of 5 by 2.
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Datasheets

    • NGFR p75 Antibody (MC-192) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against PC-12 cells of rat origin
    • recommended for detection of NGFR p75 of mouse and rat origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See NGFR p75 (B-1): sc-271708 for NGFR p75 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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which amino acid sequence was the antibody raised for?

Asked by: Cassiano
Thank you for your question. NGFR p75 Antibody (MC-192): sc-53631 was raised against PC-12 cells of rat origin (accession number P07174). No epitope mapping has been performed for this antibody, so the exact epitope sequence is unknown. If you have further questions please feel free to contact our technical service team directly.
Answered by: Technical Support Europe
Date published: 2019-04-05

Can NGFR p75 (MC-192): sc-53631 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, NGFR p75 (MC-192): sc-53631 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Published using this antibody in our Western Published using this antibody in our Western Blot against human cell lines.
Date published: 2015-08-24
Rated 4 out of 5 by from Very large WB band obtained using PC Very large WB band obtained using PC-12 whole cell lysate under non-reducing conditions. -SCBT QC
Date published: 2013-06-05
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