NGFR p75 Antibody (B-1)recommended for detection of NGFR p75 of mouse, rat and human origin by WB, IP, IF and ELISA

NGFR p75 Antibody (B-1): sc-271708

NGFR p75 Antibody (B-1) is rated 4.8 out of 5 by 13.
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Datasheets

    • NGFR p75 Antibody (B-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 393-427 at the C-terminus of NGFR p75 of human origin
    • NGFR p75 Antibody (B-1) is recommended for detection of NGFR p75 of mouse, rat and human origin by WB, IP, IF and ELISA
    • NGFR p75 Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-271708 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NGFR p75 (B-1): sc-271708.
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Hi! I wonder which is the best method for WB analysis. In particular which blocking solution do you use?

Asked by: vale89
Thank you for your question. We recommend using a blocking buffer of 5% milk TBST for Western Blot. Our complete Western Blot protocol can be found on the website here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Service
Date published: 2017-07-13

What is the recommended fixation method for IF with NGFR p75 (B-1): sc-271708 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 8
Date published: 2017-02-03
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Rated 4 out of 5 by from For me it's ok The antibody anti-NGFR p75 (B-1; sc-271708) was tested for Western blotting. The samples analyzed derive from human cancer cells known to be positive or negative for the p75 expression. As shown in the western blot, the antibody recognizes two bands: a specific band corresponding to 75 KDa (as expected) and an aspecific band at about 100 KDa (as expected). Western blot conditions: Blocking 5% Milk; Ab I 1:500 in tTBS Ab II 1:2000 anti-mouse in 3% Milk
Date published: 2019-05-31
Rated 4 out of 5 by from IF on mouse lung works fine Immunofluorescence staining on mouse lung works fine with this ab.
Date published: 2018-09-19
Rated 4 out of 5 by from Clear staining in western blot I used this antibody on primary brain culture and it works very well in western blot even at low concentrations (1:2000)
Date published: 2018-05-25
Rated 5 out of 5 by from Amazing Western blot and IHC results I tried several anti-p75NGFR antibodies (also produced by different companies). However, this antibody is became my first choice. We tested sc-271708 on rat brain and human tissues, and it gives a clear and strong signal at 75 kDa, which corresponds to the mature glycosylated form of the receptor. A band at about 60 kDa is also present, corresponding to the immature form of the receptor. Depending on the physiopathological conditions, it is also possible to observe p75 cleavage products.
Date published: 2017-07-14
Rated 5 out of 5 by from excellent antibody I bought this antibody use for pig and rat peripheral nervous tissue cryosections immunofluorescence stain. We got perfect image results.
Date published: 2017-05-19
Rated 5 out of 5 by from worked well Signal is relatively strong detected by WB at 1:100 ratio (Wnt-3: 5%skim milk) in PC12 cells.
Date published: 2017-04-13
Rated 5 out of 5 by from nice It's very clear in the Western blot and a excellent antibody.
Date published: 2017-03-29
Rated 5 out of 5 by from Good NGFR p75 detection Strong signal observed in Western blot and IF for NGFR p75.
Date published: 2017-02-08
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