NGAL Antibody (5G5) recommended for detection of native and denatured forms of NGAL of human origin by WB, IF and ELISA

NGAL Antibody (5G5): sc-57517

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Datasheets

    • NGAL Antibody (5G5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 100 µg/ml
    • raised against full length native NGAL isolated from neutrophils of human origin
    • recommended for detection of native and denatured forms of NGAL of human origin by WB, IF and ELISA

I am using NGAL (5G5): sc-57517 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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