Neutrophil Elastase Antibody (G-2)recommended for detection of Neutrophil Elastase of human origin by WB, IP, IF, IHC(P) and ELISA

Neutrophil Elastase Antibody (G-2): sc-55549

Neutrophil Elastase Antibody (G-2) is rated 4.5 out of 5 by 8.
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Datasheets

    • Neutrophil Elastase Antibody (G-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 211-267. of Neutrophil Elastase of human origin
    • Neutrophil Elastase Antibody (G-2) is recommended for detection of Neutrophil Elastase of human origin by WB, IP, IF, IHC(P) and ELISA
    • Neutrophil Elastase Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Neutrophil Elastase (G-2): sc-55549.
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Which antigen retrival method you tried with that specific antibody? Did it work with Tris-EDTA pH 9.0?

Asked by: Soff00
Thank you for your question. We recommend performing antigen retrieval with sodium citrate, with heat. You can find the specifics in our protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-08-14

How can Neutrophil Elastase (G-2): sc-55549 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: cjMara
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, Neutrophil Elastase (G-2), is currently available conjugated to phycoerythrin (sc-55549 PE), fluorescein (sc-55549 FITC), Alexa Fluor® 488 (sc-55549 AF488) or Alexa Fluor® 647 (sc-55549 AF647)
Answered by: Technical Service 11
Date published: 2017-01-22

Can you recommend a positive control to use with Neutrophil Elastase (G-2): sc-55549 monoclonal antibody for Western blot?

Asked by: jenni
We recommend U-937 (sc-2239) or HL-60 (sc-2209) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Neutrophil Elastase in these cell lines.
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 5 out of 5 by from good antibody recognise human and mouse neutrophil Neutrophil Elastase expressed in blood film of transgenic mouse suffered from systemic inflammation. Neutrophil Elastase expressed in the activated neutrophil in the lysosomes. Azurophil granules stained with alexa 488. Very good antibody recognise human and mouse neutrophil.
Date published: 2017-02-17
Rated 5 out of 5 by from Superb !! Oustanding western blot data; specific band; also strong IHC
Date published: 2017-01-29
Rated 1 out of 5 by from IHC (P): no staining of neutrophils The antibody does not react with neutrophils in normal human spleen paraffin sections (fixation by 3.7% formol in tap water for 24h). Antigen retrievals tested were: 1) autoclaving in citrate buffer pH 6.0; 2) protease XIV; 3) no pretreatment.
Date published: 2017-01-18
Rated 5 out of 5 by from Excellent antibody The Neutrophil Elastase Antibody (G-2): sc-55549 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from Used for immunohistochemical analysis of plasma Used for immunohistochemical analysis of plasma, ascitic fluid, and pancreas sections from male wistar rats. -SCBT Publication Review
Date published: 2015-04-24
Rated 5 out of 5 by from Robust bands observed at the correct molecular Robust bands observed at the correct molecular weight in western blot analysis of Neutrophil Elastase expression in U-937 and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Immunofluorescence with pfa fixed rat brain Immunofluorescence with pfa fixed rat brain sections. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic staining Superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human bone marrow tissue. -SCBT QC
Date published: 2015-01-17
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