Neutrophil Elastase Antibody (F-1)recommended for detection of Neutrophil Elastase of human origin by WB, IP, IF and ELISA

Neutrophil Elastase Antibody (F-1): sc-55548

Neutrophil Elastase Antibody (F-1) is rated 5.0 out of 5 by 2.
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Datasheets

    • Neutrophil Elastase Antibody (F-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 211-267 of Neutrophil Elastase of human origin
    • recommended for detection of Neutrophil Elastase of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Neutrophil Elastase (G-2): sc-55549 for Neutrophil Elastase antibody conjugates, including AC, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
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What is the recommended starting dilution for Western blot for sc-55548: Neutrophil Elastase (F-1) monoclonal antibody?

Asked by: jenni
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of Neutrophil Elastase expression in U-937 and HL-60 whole cell lysates.
Date published: 2017-02-03
Rated 5 out of 5 by from Great Western blot data of Neutrophil Elastase Great Western blot data of Neutrophil Elastase expression in U-937 and HL-60 whole cell lysates. -SCBT QC
Date published: 2013-11-12
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