Anti-Neutrophil Elastase Antibody (201) recommended for detection of Neutrophil Elastase of human origin by WB, IP and ELISA

Anti-Neutrophil Elastase Antibody (201): sc-59335

Anti-Neutrophil Elastase Antibody (201) is rated 4.0 out of 5 by 1.
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Datasheets

    • Neutrophil Elastase Antibody (201) is a mouse monoclonal IgM provided at 100 µg/ml
    • raised against full length native Neutrophil Elastase of human origin
    • recommended for detection of Neutrophil Elastase of human origin by WB, IP and ELISA
    • See Neutrophil Elastase (G-2): sc-55549 for Neutrophil Elastase antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Neutrophil Elastase Antibody (201). This work is in progress.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with Neutrophil Elastase (201): sc-59335 antibody?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Very clean Very clean, strong bands in western blot analysis of Neutrophil Elastase expression in HL-60, U-937, LADMAC, human PBL, AML-193 and NCI-H1688 whole cell lysates. -SCBT QC
Date published: 2014-10-11
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