Anti-nestin Antibody (Rat-401) recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-nestin Antibody (Rat-401): sc-33677

Anti-nestin Antibody (Rat-401) is rated 4.2 out of 5 by 13.
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Datasheets

    • nestin Antibody (Rat-401) is a mouse monoclonal IgG1 κ, cited in 83 publications, provided at 200 µg/ml
    • raised against spinal cord extracts of embryonic rat
    • Anti-nestin Antibody (Rat-401) is recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-nestin Antibody (Rat-401) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to PerCP for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of nestin (Rat-401): sc-33677.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for nestin Antibody (Rat-401) for WB and IHC(P) applications. These reagents are now offered in bundles with nestin Antibody (Rat-401) (see ordering information below).
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Is this the same clone as http://dshb.biology.uiowa.edu/nestin?gclid=Cj0KCQjw-uzVBRDkARIsALkZAdkZSLcQYBQlgqD83lLpxUAFHLN6XzADx1_Kpin-nkjkAyvWZffpoYcaAs0wEALw_wcB ? Many suppliers sell Rat 401 and I am confused. 

Asked by: 4DNCWG
Thank you for your question. Yes, this nestin mouse monoclonal antibody with clone designation Rat-401 is commercially available from other suppliers as well as Santa Cruz Biotechnology.
Answered by: Technical Support
Date published: 2019-06-16

I can not find a single reference where this antibody is used for immunoprecipitation. Do you have evidence that antibody (Rat-401) can be used for such a purpose?

Asked by: ChrisB
Thank you for your question. We are not aware of any publications citing use of this nestin Antibody (Rat-401): sc-33677 for immunoprecipitation. The antibody has been cited in over 40 publications, but is most commonly used for WB, IF and IHC applications. We do warranty the antibody for IP based on the antibody's performance in other research applications. If you purchase the antibody, and it does not work in any of the recommended applications, please contact our Technical Service Department. We can assist you with troubleshooting the antibody, and offer either a replacement or refund of your purchase.
Answered by: Technical Support
Date published: 2017-02-23

What is the recommended secondary to use with nestin Antibody (Rat-401): sc-33677?

Asked by: jenni
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 1 out of 5 by from Not very good I tried the Ab Nestin rat-401in human neuronal progenitor cells by IF (1:200) Doesn't work very well.
Date published: 2018-02-13
Rated 5 out of 5 by from Good Antibody nestin Antibody prove helpful and efficient. It is also able to improve rat's experiment enhance sensitivity.It's our ideal choice to do drugtest.
Date published: 2017-10-18
Rated 3 out of 5 by from Nestin (Rat 401) I tried an aliquot of this antibody and it has been working fine.
Date published: 2017-10-13
Rated 1 out of 5 by from Does not work in horse We tried this antibody to immunolabel horse samples, but were unable to visualise any staining, despite trying different staining conditions. We are now using a nestin antibody from another supplier.
Date published: 2017-09-19
Rated 5 out of 5 by from great quality Very nice and strong antibody. Excellent to determine nestin in rat kidney tissue extracts,1:1000 diluted the antibody.
Date published: 2017-07-22
Rated 5 out of 5 by from WB dilution in case of 1:500 work Often use this antibody nestin, WB dilution in the case of 1:500 work
Date published: 2017-06-05
Rated 5 out of 5 by from positive Good detection in rat embryonic brain tissue extract in WB, dilution this antibody at 1:200.
Date published: 2017-05-10
Rated 5 out of 5 by from very recommended The concentration of nestin antibody in WB diluted with 1:1000 can be satisfied with the band
Date published: 2017-04-10
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