Anti-nestin Antibody (D-9) recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-nestin Antibody (D-9): sc-377380

Anti-nestin Antibody (D-9) is rated 4.8 out of 5 by 4.
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Datasheets

    • nestin Antibody (D-9) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 235-320 of nestin of human origin
    • Anti-nestin Antibody (D-9) is recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-nestin Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of nestin (D-9): sc-377380.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for nestin Antibody (D-9). This work is in progress.
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I am using nestin (D-9): sc-377380 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great antibody I've used this antibody for WB and it worked very good. I recommend it
Date published: 2018-01-08
Rated 5 out of 5 by from Beautiful IHC(P) results This nestin antibody gives beautiful staining data in formalin fixed, paraffin-embedded human kidney tissue showing membrane staining of cells in glomeruli.
Date published: 2017-02-03
Rated 4 out of 5 by from Positive signal achieved in IMR Positive signal achieved in IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by from Produced excellent immunoperoxidase membrane Produced excellent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-01-19
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