Anti-nestin Antibody (2Q178) recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-nestin Antibody (2Q178): sc-58813

Anti-nestin Antibody (2Q178) is rated 4.7 out of 5 by 3.
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Datasheets

    • nestin Antibody (2Q178) is a mouse monoclonal IgG1 κ, cited in 17 publications, provided at 200 µg/ml
    • raised against full length native nestin protein purified from embryonic spinal cord of rat origin
    • recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • See nestin (Rat-401): sc-33677 for nestin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for nestin Antibody (2Q178) for WB and IHC(P) applications. This reagent is now offered in a bundle with nestin Antibody (2Q178) (see ordering information below).
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I want an antibody to detect nestin by IHC(P) in human sections. The datasheet is contradictory: "recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; non cross-reactive with human nestin". Any explanations?

Asked by: Susan2706
Thank you for your question. This antibody, nestin (2Q178): sc-58813, is reactive with human and is recommended for IHC(P). We apologize for the conflicting information on our product data sheet. We will update the information on the website and data sheet right away.
Answered by: Technical Support
Date published: 2017-06-27

I am using nestin (2Q178): sc-58813 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Worked well with mESCs by IF To detect the expression of Nestin in mESCs in the treatment with RA by IF, I bought this antibody. The cells were incubated with sc-58813 at a 1/300 dilution overnight at +4°C, and found this antibody worked well.
Date published: 2016-12-06
Rated 5 out of 5 by from Works great in Western Blot Works great in Western Blot, will buy it again
Date published: 2015-03-24
Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmic Produced exceptional immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-02-24
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