Anti-nestin Antibody (2C1.3A11) recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-nestin Antibody (2C1.3A11): sc-73489

Anti-nestin Antibody (2C1.3A11) is rated 3.5 out of 5 by 2.
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Datasheets

    • nestin Antibody (2C1.3A11) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against a 150 amino acid epitope mapping near the C-terminus of human nestin
    • recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See nestin (10c2): sc-23927 for nestin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for nestin Antibody (2C1.3A11) for WB and IHC(P) applications. These reagents are now offered in bundles with nestin Antibody (2C1.3A11) (see ordering information below).
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I am using nestin (2C1.3A11): sc-73489 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent features I am happy I purchase this antibody. It was very stable and the result could be repeated.I will continue to use it.
Date published: 2017-02-13
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