Anti-Nectin 3 Antibody (H-11) recommended for detection of Nectin 3 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Nectin 3 Antibody (H-11): sc-271611

Anti-Nectin 3 Antibody (H-11) is rated 4.8 out of 5 by 6.
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Datasheets

    • Anti-Nectin 3 Antibody (H-11) is a mouse monoclonal IgG1 (kappa light chain) Nectin 3 antibody provided at 200 µg/ml
    • raised against amino acids 301-545 mapping near the C-terminus of Nectin 3 of human origin
    • Anti-Nectin 3 Antibody (H-11) is recommended for detection of Nectin 3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Nectin 3 Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nectin 3 (H-11): sc-271611.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Nectin 3 Antibody (H-11) for WB applications. These reagents are now offered in bundles with Nectin 3 Antibody (H-11) (see ordering information below).
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Are there any issues with using sc-271611: Nectin 3 (H-11) mouse monoclonal primary antibody on mouse samples?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-271611 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Great antibody Works very well in human neural stem cells. Visible band at 100 kDa.
Date published: 2017-12-14
Rated 5 out of 5 by from Nectin-3 antibody Excelent antibody for western blot. Detected Nectin-3 at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti Nectin-3 in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 5 out of 5 by from very satisfied WB investigate the expression of Nectin 3 in breast cancer tissues ,very satisfied with strong signal at 83kda,use the dilution 1:800.
Date published: 2017-09-03
Rated 4 out of 5 by from good quality antibody Nectin 3 antibody is a good antibody for detection of Nectin 3 by WB (1:800, 5% BSA,TBST),we got specific band, but the IF result have some background signal,the condition should be optimized.
Date published: 2017-07-29
Rated 5 out of 5 by from gave strong signal This antibody gave strong signal in Hepg2 cell lysates ,I got target band. Dilution was 1:1000 and there were no visibility of background signals.
Date published: 2017-07-08
Rated 5 out of 5 by from Produced nice Western blot data of Nectin Produced nice Western blot data of Nectin 3 expression in 3T3-L1 whole cell lysate. -SCBT QC
Date published: 2014-06-26
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