Anti-Nectin 1 Antibody (R1.302.12) recommended for detection of Nectin 1 of human origin by WB, IP, IF, IHC(P) and FCM

Anti-Nectin 1 Antibody (R1.302.12): sc-69718

Anti-Nectin 1 Antibody (R1.302.12) is rated 4.0 out of 5 by 2.
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Datasheets

    • Nectin 1 Antibody (R1.302.12) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
    • raised against NIH3T3 cells transfected with Nectin 1 of human origin
    • recommended for detection of Nectin 1 of human origin by WB, IP, IF, IHC(P) and FCM; may cross-react with Nectin 1 of monkey origin; non cross-reactive with Nectin 1 of mouse and rat origin
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See Nectin 1 (CK6): sc-21722 for Nectin 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Nectin 1 Antibody (R1.302.12) for WB and IHC(P) applications. These reagents are now offered in bundles with Nectin 1 Antibody (R1.302.12) (see ordering information below).
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Can Nectin 1 (R1.302.12): sc-69718 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Nectin 1 (R1.302.12): sc-69718 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from ELISA publishable data with hamster ELISA publishable data with hamster,murine,green monkey,human J1.1-2 cells ,B78H1 cells,ATCC CCL-81, VD60 cells ,HT29 cells, BxPC3 cells - SCBT Publication Review
Date published: 2015-04-17
Rated 4 out of 5 by from Produced wonderful FCM data for Jurkat cells Produced wonderful FCM data for Jurkat cells. -SCBT QC
Date published: 2014-01-04
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