NDUFS2 Antibody (B-3)recommended for detection of NDUFS2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

NDUFS2 Antibody (B-3): sc-390596

NDUFS2 Antibody (B-3) is rated 4.8 out of 5 by 4.
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Datasheets

    • NDUFS2 Antibody (B-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 101-344 mapping within an internal region of NDUFS2 of human origin
    • NDUFS2 Antibody (B-3) is recommended for detection of NDUFS2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • NDUFS2 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NDUFS2 (B-3): sc-390596.
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Can NDUFS2 (B-3): sc-390596 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, NDUFS2 (B-3): sc-390596 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from very good positive results I have an expected result that has been detected at 1/1000 and this antibody has provided us with a target band of 49 kDa.
Date published: 2017-11-01
Rated 4 out of 5 by from Good signal! Good in Western Blot, but the band is lower than the expected size...
Date published: 2017-09-28
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic staining Great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Great Western Blot data of NDUFS2 expression Great Western Blot data of NDUFS2 expression in Jurkat, CCRF-CEM, A431 and HeLa whole cell lysates. -SCBT QC
Date published: 2014-10-07
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