NDUFS1 Antibody (E-8)recommended for detection of NDUFS1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

NDUFS1 Antibody (E-8): sc-271510

NDUFS1 Antibody (E-8) is rated 5.0 out of 5 by 6.
  • y_2019, m_12, d_6, h_22
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_5
  • loc_en_US, sid_271510, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 50ms
  • REVIEWS, PRODUCT
5
6
4
0
3
0
2
0
1
0

Datasheets

    • NDUFS1 Antibody (E-8) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 428-727 mapping at the C-terminus of NDUFS1 of human origin
    • NDUFS1 Antibody (E-8) is recommended for detection of NDUFS1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • NDUFS1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NDUFS1 (E-8): sc-271510.
Submit a review for this product and receive 15 CruzCredits

I am using NDUFS1 (E-8): sc-271510 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2019, m_12, d_6, h_22CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_271510, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 54ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great antibody! Used it in Western Blot, strong and clean signal. Works better on nitrocellulose membrane than PVDF.
Date published: 2017-09-28
Rated 5 out of 5 by from Good antibody I got the sample of NDUFS1 antibody (E-8) about 2 months ago. The antibody detected the band whose size is supposed to be NDUFS1 in 293T cells, and was very clean. When I transfected 293T cells with empty vector or NDUFS1 expression vector, WB with this antibody clearly showed that cells transfected with NDUFS1 expression vector had much higher levels than the cells transfected with empty vector.
Date published: 2017-05-02
Rated 5 out of 5 by from excellent for WB strong, clear, low backgroud band was visualised in human cell lysate.
Date published: 2017-04-12
Rated 5 out of 5 by from Worked well in WB I used this antibody in WB (dilution 1:1000), the band is wonderful. No issue at all.
Date published: 2016-12-09
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-05-06
  • y_2019, m_12, d_6, h_22
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_5
  • loc_en_US, sid_271510, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 35ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.