NDRG1 Antibody (B-5)recommended for detection of NDRG1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

NDRG1 Antibody (B-5): sc-398291

NDRG1 Antibody (B-5) is rated 4.0 out of 5 by 5.
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Datasheets

    • NDRG1 Antibody (B-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-29 at the N-terminus of NDRG1 of human origin
    • NDRG1 Antibody (B-5) is recommended for detection of NDRG1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • NDRG1 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-398291 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NDRG1 (B-5): sc-398291.
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What application is the blocking peptide sc-398291 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great signal in hypoxic cells I have used this antibody for hypoxia experiments. I obtained an excellent specific signal particularly in hypoxic cancer cells by western blotting. Really happy with this antibody.
Date published: 2019-11-18
Rated 1 out of 5 by from Does not work well for detection of HEK293 cells Very faint signal at ~46 KD detected from HEK293 cells. A strong band shows up at ~50 KD, but unfortunately, this is not the NDRG1 protein, because the anti NDRG1-p does not have a signal in this position.
Date published: 2018-09-11
Rated 4 out of 5 by from Good for WB This antibody worked well for WB in non-transfected HepG2 cell.
Date published: 2017-05-11
Rated 5 out of 5 by from Produced great immunofluorescence cytoskeletal Produced great immunofluorescence cytoskeletal staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Good Western Blot data of NDRG1 expression in non Good Western Blot data of NDRG1 expression in non-transfected 293T, human NDRG1 transfected 293T and LNCaP whole cell lysates. -SCBT QC
Date published: 2013-03-13
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