Anti-NDH II Antibody (B-9) recommended for detection of nuclear DNA helicase II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-NDH II Antibody (B-9): sc-137232

Anti-NDH II Antibody (B-9) is rated 4.2 out of 5 by 5.
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Datasheets

    • Anti-NDH II Antibody (B-9) is a mouse monoclonal IgG2b κ NDH II antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of NDH II of human origin
    • Anti-NDH II Antibody (B-9) is recommended for detection of nuclear DNA helicase II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-NDH II Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-137232 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NDH II (B-9): sc-137232.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for NDH II Antibody (B-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with NDH II Antibody (B-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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When should the TransCruz reagent sc-137232 X be used instead of the standard form sc-137232?

Asked by: cjMara
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Acceptable Not very strong signals and some background bands when used in western blot of total human (Jurkat, K562, HL-60) cell extracts.
Date published: 2021-05-31
Rated 3 out of 5 by from 为什么此抗体在不同细胞中WB出现不同的条带数量 我们用此抗体做WB时在返修中被编辑针对不同条带数量的问题整惨了。。。 此抗体1:1000稀释目的条带容易显影
Date published: 2019-10-09
Rated 5 out of 5 by from Excellent antibody for CoIP CoIP was successfully done with this antibody at 1:500. The bands were recognized around 130 KD.
Date published: 2017-05-11
Rated 5 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Produced positive Western Blot data of NDH Produced positive Western Blot data of NDH II expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-06-06
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