Anti-N-Myc Antibody (NCM II 100) recommended for detection of N-myc encoded proteins and their cleavage products of mouse, rat and human origin by WB, IP and IF

Anti-N-Myc Antibody (NCM II 100): sc-56729

Anti-N-Myc Antibody (NCM II 100) is rated 4.6 out of 5 by 5.
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Datasheets

    • N-Myc Antibody (NCM II 100) is a mouse monoclonal IgG1 κ, cited in 30 publications, provided at 200 µg/ml
    • raised against recombinant N-Myc of human origin
    • Anti-N-Myc Antibody (NCM II 100) is recommended for detection of N-myc encoded proteins and their cleavage products of mouse, rat and human origin by WB, IP and IF
    • Anti-N-Myc Antibody (NCM II 100) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of N-Myc (NCM II 100): sc-56729.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for N-Myc Antibody (NCM II 100) for WB applications. These reagents are now offered in bundles with N-Myc Antibody (NCM II 100) (see ordering information below).
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Which residues does this N-Myc antibody recognizes?

Asked by: gsegat
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody have not yet been determined.
Answered by: Technical Support
Date published: 2020-05-07

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?
Answered by: Technical Service 11
Date published: 2021-01-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-11
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Rated 3 out of 5 by from Works OK We used it for IF, and it works OK in neuroblastoma cell lines, which have high endogenous N-Myc protein levels.. Not as good as B4.8.B though.
Date published: 2019-08-14
Rated 5 out of 5 by from Validated MYCN antibody for FFPE IHC use MYCN II 100 antibody was raised against the second exon of MYCN (Proc Natl Acad Sci USA. 1986 Aug;83(16):5929–33.) and used to stain a large cohort of neuroblastomas (Br J Cancer. 2015 Jun 30;113(1):57–63). MYCN is expressed in the skin hair follicles (Developmental Cell. 2005 Aug;9(2):293–303) and in 30% of basal cell carcinomas (Acta Derm Venereol. 2019 Jan 1;99(1):63–71).
Date published: 2019-07-14
Rated 5 out of 5 by from Versatile antibody! The N-Myc Antibody (NCM II 100): sc-56729 works very well for multiple applications including WB, IP, and IF. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from Good WB with 3T3 Good WB with 3T3-MycER fibroblasts, ES cells. -SCBT Publication Review
Date published: 2012-08-28
Rated 5 out of 5 by from Used IGR Used IGR-N-91 cell line in Western Blot -SCBT Publication Review
Date published: 2012-02-04
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