Anti-myogenin Antibody (F5D) recommended for detection of myogenin of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-myogenin Antibody (F5D): sc-12732

Anti-myogenin Antibody (F5D) is rated 4.8 out of 5 by 19.
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Datasheets

    • myogenin Antibody (F5D) is a mouse monoclonal IgG1 κ, cited in 346 publications, provided at 200 µg/ml
    • epitope mapping within amino acids 138-158 of myogenin of rat origin
    • Anti-myogenin Antibody (F5D) is recommended for detection of myogenin of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • myogenin Antibody (F5D) is available conjugated to agarose for IP
    • also available conjugated to Alexa Fluor® 594 for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-12732 X, 200 µg/0.1 ml)
    • See myogenin (5FD): sc-52903 for myogenin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of myogenin (F5D): sc-12732.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for myogenin Antibody (F5D) for WB and IHC(P) applications. These reagents are now offered in bundles with myogenin Antibody (F5D) (see ordering information below).
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I need a secondary reagent to be used with myogenin Antibody (F5D): sc-12732 mouse monoclonal antibody by Western blot? I will be using the Licor system. Do you have a recommendation?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins CFL 680 or CFL 790 as a secondary detection reagent. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2016-12-15

Can myogenin Antibody (F5D): sc-12732 be used with my pig samples?

Asked by: jenni
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Santa Cruz TS
Date published: 2016-12-14
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Rated 5 out of 5 by from beautiful IF result This is good Antibody. Cell type: PMi28 (murine skeletal cells) Conc: 1μg/ml 4% PFA fix
Date published: 2019-08-26
Rated 5 out of 5 by from works fine we were given a free sample to try. It works just fine. It could detect as least as 5 ug of WCL loaded. And I re-used the diluted antibody for at least 5-8 times (kept the diluted AB at -20oC). For WB TBST was used, for blocking I used 5% milk in TBST.
Date published: 2018-08-27
Rated 4 out of 5 by from Good signal This antibody works well on human and mouse samples in WB
Date published: 2018-08-01
Rated 5 out of 5 by from Great product We used this antibody for western blot. It works for us.
Date published: 2017-09-27
Rated 4 out of 5 by from Great features! The antibody is work well in WB at 1:100,skeletal muscle cells.
Date published: 2017-09-01
Rated 5 out of 5 by from good result We have used it for WB and IF of 293 cells.WB 1:1000,IF 1:100,very good result ,sensitve and specific.
Date published: 2017-08-19
Rated 5 out of 5 by from great effective antibody I have been using this antibody (and a few others) from santa cruz for the past 3 years. I have always had good staining and clear bands. Furthermore, the price is quite competitive.
Date published: 2017-07-28
Rated 5 out of 5 by from Great antibody Great antibody for Western detection ,should be optimized for 1 hour primary incubation or 4℃ overnight,also the dilution should be optimized from 1:600 to 1:1000.
Date published: 2017-07-08
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