Anti-myogenin Antibody (5FD) recommended for detection of myogenin of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-myogenin Antibody (5FD): sc-52903

Anti-myogenin Antibody (5FD) is rated 4.8 out of 5 by 13.
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Datasheets

    • Anti-myogenin Antibody (5FD) is a mouse monoclonal IgG1 κ myogenin antibody, cited in 65 publications, provided at 200 µg/ml
    • raised against amino acids 144-158 of myogenin of rat origin
    • recommended for detection of myogenin of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • available conjugated to HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of myogenin (5FD): sc-52903.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for myogenin Antibody (5FD) for WB and IHC(P) applications. This reagent is now offered in a bundle with myogenin Antibody (5FD) (see ordering information below).
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Can you recommend a positive control to use with sc-52903: myogenin (5FD) for Western blot?

Asked by: jenniferc
We recommend SJRH30: sc-2287 or RD: sc-364791 whole cell lysates as we have great Western blot data showing specific detection of myogenin in these cell lines.
Answered by: Technical Support 7
Date published: 2017-01-10
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Rated 4 out of 5 by from band in the right size I got this sample and tried it using western blot and it working BUT a second higher band was there too
Date published: 2018-03-15
Rated 5 out of 5 by from Worked very well for western blotting Received a free sample of this antibody a month back to test on differentiated L6 cells. The antibody worked very well at a 1:200 dilution in 5% milk in TBS for western blotting using 30ug of cell lysate per well.
Date published: 2017-03-06
Rated 4 out of 5 by from C2C12 experiment we detected the band in Western blot analysis. sensitive not very good
Date published: 2017-02-20
Rated 4 out of 5 by from specific but cross-reacts other muscle proteins Myogenin antibody is specific for myogenin but also cross-reacts with a number of other muscle proteins
Date published: 2017-02-19
Rated 5 out of 5 by from Highly specific, great antbody I first tried free sample of this antibody and was happy with the result. Then I ordered it. Single band in Western blot. Love it.
Date published: 2017-01-31
Rated 5 out of 5 by from very good the antibody is very specific. the band is very strong! worth to use it!
Date published: 2017-01-05
Rated 5 out of 5 by from Versatile antibody! This antibody works great for a number of applications with verification in multiple publications for Western blot and IHC.
Date published: 2016-12-16
Rated 5 out of 5 by from Satisfied IF result We got this product 2 weeks ago,performming IF in PBS with 1:100 dilution overnight.As a result,strong singnal had been made in our lab.
Date published: 2016-11-03
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