MYL2 Antibody (7C9): sc-517244
- MYL2 Antibody (7C9) is a mouse monoclonal IgG1 κ MYL2 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 2-166 of MYL2 of human origin
- MYL2 Antibody (7C9) is recommended for detection of MYL2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-MYL2 Antibody (7C9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MYL2 Antibody (7C9). This work is in progress.
MYL2 Antibody (7C9) is a mouse monoclonal IgG1 kappa light chain antibody that detects MYL2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-MYL2 antibody (7C9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MYL2 protein, also known as myosin regulatory light chain 2, ventricular/cardiac muscle isoform, plays a crucial role in cardiac muscle contraction by regulating the interaction between myosin and actin filaments in the heart. This regulation is vital for maintaining proper cardiac function and contractility, as MYL2 allows for precise control of muscle contraction in response to calcium levels. MYL2 protein is predominantly expressed in adult cardiac ventricle muscle, where MYL2 forms part of a hexameric complex with two heavy chains and four light chains. Notably, co-expression of MYL2 with myosin regulatory light chain 7 (MYL7) in specific regions of the heart aids in delineating cardiac chamber boundaries, which is essential for normal heart development and function. Additionally, mutations in MYL2 gene have been linked to mid-left ventricular chamber type hypertrophic cardiomyopathy and familial hypertrophic cardiomyopathy type 10, highlighting MYL2′s importance in cardiovascular health. At the amino acid level, there is a remarkable 96% homology between human and mouse MYL2 sequences, making MYL2 (7C9) antibody a valuable tool for studying cardiac function across different species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MYL2 Antibody (7C9) References:
- The murine atrial myosin light chain-2 gene: a member of an evolutionarily conserved family of contractile proteins. | Doevendans, PA., et al. 2000. Cytogenet Cell Genet. 90: 248-52. PMID: 11124527
- An extra human chromosome 21 reduces mlc-2a expression in chimeric mice and Down syndrome. | Nishigaki, R., et al. 2002. Biochem Biophys Res Commun. 295: 112-8. PMID: 12083776
- Functions of myosin light chain-2 (MYL2) in cardiac muscle and disease. | Sheikh, F., et al. 2015. Gene. 569: 14-20. PMID: 26074085
- Hypertrophic remodelling in cardiac regulatory myosin light chain (MYL2) founder mutation carriers. | Claes, GR., et al. 2016. Eur Heart J. 37: 1815-22. PMID: 26497160
- Novel frameshift variant in MYL2 reveals molecular differences between dominant and recessive forms of hypertrophic cardiomyopathy. | Manivannan, SN., et al. 2020. PLoS Genet. 16: e1008639. PMID: 32453731
- LncRNA-MYL2-2 and miR-124-3p Are Associated with Perioperative Neurocognitive Disorders in Patients after Cardiac Surgery. | Xiao, QX., et al. 2021. J Invest Surg. 34: 1297-1303. PMID: 32727232
- Poor Myocardial Compaction in a Patient with Recessive MYL2 Myopathy. | Tamamitsu, AM., et al. 2021. Int Heart J. 62: 445-447. PMID: 33731536
- MYL2 as a potential predictive biomarker for rhabdomyosarcoma. | Wang, J., et al. 2021. Medicine (Baltimore). 100: e27101. PMID: 34596111
- Not All Left Ventricular Hypertrophy Is Hypertrophic Cardiomyopathy: MYL2-Associated Double-Chambered Left Ventricle. | Garcia Brás, P., et al. 2023. Can J Cardiol. 39: 1332-1334. PMID: 37257752
- Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis. | Kubalak, SW., et al. 1994. J Biol Chem. 269: 16961-70. PMID: 8207020
- Downregulation of atrial markers during cardiac chamber morphogenesis is irreversible in murine embryos. | Gruber, PJ., et al. 1998. Development. 125: 4427-38. PMID: 9778502
- Myosin light chain 2a and 2v identifies the embryonic outflow tract myocardium in the developing rodent heart. | Franco, D., et al. 1999. Anat Rec. 254: 135-46. PMID: 9892427
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYL2 Antibody (7C9) | sc-517244 | 200 µg/ml | $322.00 | |||
MYL2 Antibody (7C9) AC | sc-517244 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MYL2 Antibody (7C9) HRP | sc-517244 HRP | 200 µg/ml | $322.00 | |||
MYL2 Antibody (7C9) FITC | sc-517244 FITC | 200 µg/ml | $336.00 | |||
MYL2 Antibody (7C9) PE | sc-517244 PE | 200 µg/ml | $349.00 | |||
MYL2 Antibody (7C9) Alexa Fluor® 488 | sc-517244 AF488 | 200 µg/ml | $364.00 | |||
MYL2 Antibody (7C9) Alexa Fluor® 546 | sc-517244 AF546 | 200 µg/ml | $364.00 | |||
MYL2 Antibody (7C9) Alexa Fluor® 594 | sc-517244 AF594 | 200 µg/ml | $364.00 | |||
MYL2 Antibody (7C9) Alexa Fluor® 647 | sc-517244 AF647 | 200 µg/ml | $364.00 | |||
MYL2 Antibody (7C9) Alexa Fluor® 680 | sc-517244 AF680 | 200 µg/ml | $364.00 | |||
MYL2 Antibody (7C9) Alexa Fluor® 790 | sc-517244 AF790 | 200 µg/ml | $364.00 |