MYH7 Antibody (A4.951)recommended for detection of myosin heavy chain 7 of mouse, rat, human, rabbit and fish origin by WB, IP, IF and IHC(P)

MYH7 Antibody (A4.951): sc-53090

MYH7 Antibody (A4.951) is rated 4.3 out of 5 by 8.
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Datasheets
    • MYH7 Antibody (A4.951) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against adult skeletal muscle myosin of human origin
    • MYH7 Antibody (A4.951) is recommended for detection of myosin heavy chain 7 of mouse, rat, human, rabbit and fish origin by WB, IP, IF and IHC(P)
    • MYH7 Antibody (A4.951) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MYH7 (A4.951): sc-53090.
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What is the recommended starting dilution for Western blot for MYH7 (A4.951)?

Asked by: cjMara
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-33686.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 8
Date published: 2017-01-12

What is the exact epitope recognized by MYH7 (A4.951): sc-53090?

Asked by: cjMara
MYH7 Antibody (A4.951) is a mouse monoclonal raised against adult skeletal muscle myosin of human origin. The exact epitope has not been determined.
Answered by: Technical Support 8
Date published: 2017-01-12
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Rated 2 out of 5 by from not so good result Using IF, strong back ground appeared. conc: 0.4 μg~4 μg/ml
Date published: 2019-08-26
Rated 5 out of 5 by from Great signal for WB I bought this on Jan, 2018 for analyzing MYH7 in iPSC-CM. The result is great.
Date published: 2018-05-30
Rated 5 out of 5 by from data Good antibody by WB(dilution 1/1000) on Human cancer cell.
Date published: 2017-04-05
Rated 4 out of 5 by from Okay Not as strong an antibody as the MYH7(A4.840) clone also available from santa cruz
Date published: 2017-01-17
Rated 5 out of 5 by from A great choice! This antibody can be expected to produce reliable detection of MYH7. Produces good WB data and even better immunostains. It's been cited several times by researchers.
Date published: 2016-12-23
Rated 5 out of 5 by from This has worked well in WB This has worked well in WB.
Date published: 2015-10-21
Rated 5 out of 5 by from Good staining of myocytes in formalin fixed Good staining of myocytes in formalin fixed, paraffin-embedded human skeletal muscle tissue in IHC -SCBT QC
Date published: 2015-05-15
Rated 3 out of 5 by from Strong signal with background seen in WB Strong signal with background seen in WB with human skeletal muscle tissue extract. -SCBT QC
Date published: 2014-05-15
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