MYH7 Antibody (A4.840)recommended for detection of myosin heavy chain 7 of mouse, rat, human, rabbit and avian origin by WB, IP, IF and IHC(P)

MYH7 Antibody (A4.840): sc-53089

MYH7 Antibody (A4.840) is rated 4.6 out of 5 by 11.
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Datasheets

    • MYH7 Antibody (A4.840) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against adult skeletal muscle myosin of human origin
    • recommended for detection of myosin heavy chain 7 of mouse, rat, human, rabbit and avian origin by WB, IP, IF and IHC(P)
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MYH7 (A4.840): sc-53089.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Does this antibody work for rat tissue? Also, what is the recommended lysis buffer for homogenization?

Asked by: NotTom
Thank you for your inquiry! Yes, this antibody is mouse, rat and human reactive, so you can use it on your rat tissues. We recommend using RIPA buffer as a lysis buffer. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-01-17

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-11

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-53089 PE), fluorescein (sc-53089 FITC), Alexa Fluor® 488 (sc-53089 AF488) or Alexa Fluor® 647 (sc-53089 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-11
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Rated 2 out of 5 by from not working I tested for SD rat aortic aortic smooth muscle cells,Regrettably,I can not got any results,Probably,it is the myh7 molecular weight is so large
Date published: 2017-07-13
Rated 4 out of 5 by from It is not very good in C2C12 WB It is not as good as MYH B5 in WB for C2C12 differentiation for 7 days. 1:500 dilution
Date published: 2017-05-31
Rated 5 out of 5 by from MYH7 antibody I have tried the MYH7 antibody in Western blot, MYH7 antibody is very good, the band is approximately at 220kDa and seems so clear.
Date published: 2017-05-15
Rated 5 out of 5 by from Superb product Great data for all applications including WB,IF and IHC
Date published: 2017-01-29
Rated 5 out of 5 by from Good antibody for WB The MYH7 Antibody (A4.840): sc-53089 provides good data for Western blot, showing clear band with minimal background.
Date published: 2017-01-13
Rated 5 out of 5 by from Well done in WB using heart tissue extracts This antibody work well in WB using heart tissue extracts of mouse.
Date published: 2016-11-16
Rated 5 out of 5 by from Robust cytoplasmic staining in paraffin Robust cytoplasmic staining in paraffin-embedded human heart muscle. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from IF: Very good nuclear and cytoplasmic staining IF: Very good nuclear and cytoplasmic staining in HeLa cells. -SCBT QC
Date published: 2015-03-09
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