MYH11 Antibody (I-5)recommended for detection of myosin heavy chain 11 isoforms SM-1 and SM-2 of mouse, rat and human origin by WB, IP, IF and IHC(P)

MYH11 Antibody (I-5): sc-65734

MYH11 Antibody (I-5) is rated 3.0 out of 5 by 3.
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Datasheets

    • MYH11 Antibody (I-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against crude smooth muscle extract from uterus myometrium of human origin
    • MYH11 Antibody (I-5) is recommended for detection of myosin heavy chain 11 isoforms SM-1 and SM-2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • MYH11 Antibody (I-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MYH11 (I-5): sc-65734.
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How can MYH11 (I-5): sc-65734 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: AbPolly
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-65734 HRP), phycoerythrin (sc-65734 PE), fluoroscein (sc-65734 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 1 out of 5 by from Did not work on embryonic mouse tissue We used this antibody (1/100 AND 1/500) on paraformaldehyde fixed, paraffin embedded embryonic mouse tissue and we could not detect a specific signal.
Date published: 2018-06-05
Rated 4 out of 5 by from Works well for Western blot applications Works well for Western blot applications with HISM whole cell lysate. -SCBT QC
Date published: 2015-05-08
Rated 4 out of 5 by from Very strong band in WB with human uterine Very strong band in WB with human uterine fundus tissue extract. -SCBT QC
Date published: 2013-08-12
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