MYH11 Antibody (G-4)recommended for detection of myosin heavy chain 11, isoforms SM1 and SM2 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA

MYH11 Antibody (G-4): sc-6956

MYH11 Antibody (G-4) is rated 4.6 out of 5 by 13.
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Datasheets

    • MYH11 Antibody (G-4) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
    • raised against full length smooth muscle myosin heavy chain of rat origin
    • MYH11 Antibody (G-4) is recommended for detection of myosin heavy chain 11, isoforms SM1 and SM2 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including and bovine and porcine
    • MYH11 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MYH11 (G-4): sc-6956.
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Hello, I am about to try the anti MYH11 antibody sc-6956, but I am a little confused from the picture in datasheet. There is a fibroblast NIH/3T3, which should be MYH11 negative. Is this antibody really specific for muscle cells myosin? Thanks.

Asked by: Jarmila
Thank you for your question. We are looking into any references we can provide to support the data in NIH/3T3 using this antibody, and will update the information on our website as soon as we can provide more information. In the meantime, we are currently offering a free 10 µg sample of this antibody, MYH11 (G-4): sc-6956, if you would be interested in testing the antibody in your samples. Please contact our Technical Service Department if you would like to request a sample.
Answered by: Technical Support
Date published: 2019-02-02

MYH11 (G-4): sc-6956 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. SH-PTP2 (B-1): sc-7384 monoclonal antibody is currently available conjugated to PE, FITC, Alexa Fluor 488 or Alexa Fluor 647.
Answered by: Technical Support 10
Date published: 2017-01-09

Hello, can you please tell me whether this Ab recognizes zebrafish myh11 (at least whether it could possibly recognize this based on sequence similarity?) Thanks.

Asked by: Calgaryfish
Thank you for your inquiry. Unfortunately we do not have any information regarding zebrafish reactivity for this MYH11 antibody and can not warranty it for that use. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2016-10-24
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Rated 5 out of 5 by from IHC-Fr is good it was good for IHC-Fr , the dilution ratio was 1:100.
Date published: 2018-07-07
Rated 5 out of 5 by from great product We used this antibody for immunostaining. it works for our experiments.
Date published: 2017-09-27
Rated 5 out of 5 by from nice antibody I used this MYH11 as 1:200 for IF staining, it was pretty good, yet the confocal results were put into our paper, thanks for SANTA.
Date published: 2017-09-14
Rated 1 out of 5 by from Not working in Gastric cancer cell lines No any specific WB band detected in gastric cancer cell lines.
Date published: 2017-06-21
Rated 5 out of 5 by from Perform a perfect deduction In order to test for contractile activity in smooth muscle, we purchased myosin, which is highly conserved and we perform a perfect deduction experiment by smooth muscle contracting on myosin light chain kinase-dependent regulation.
Date published: 2017-02-10
Rated 4 out of 5 by from Good performance in Western blot This antibody was used in WB with a 1:500 dilution, and a specific band was detected in the membrane.
Date published: 2017-01-06
Rated 5 out of 5 by from Excellent antibody. This is a great product that works well in standard applications. It has broad reactivity and will produce clear and specific signals. It has been published with over twenty times.
Date published: 2017-01-04
Rated 5 out of 5 by from Worked wonderfully Worked wonderfully!
Date published: 2015-06-04
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