MYH Antibody (B-5)recommended for detection of skeletal and cardiac myosin heavy chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

MYH Antibody (B-5): sc-376157

MYH Antibody (B-5) is rated 4.5 out of 5 by 8.
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Datasheets

    • MYH Antibody (B-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1641-1940 of myosin heavy chain 3 of human origin
    • MYH Antibody (B-5) is recommended for detection of skeletal and cardiac myosin heavy chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • MYH Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MYH (B-5): sc-376157.
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Do mouse MYH2, MYH6, MYH7 can be detected by MYH(B-5) in WB?

Asked by: SYH05
Thank you for your question. This MYH antibody, sc-376157, will for sure detect MYH3, but has not been epitope mapped so we can not guarantee that it can pick up MYH2, MYH7 or MYH6. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-10-19

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-24
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Rated 4 out of 5 by from Good Meets what was promised for WB, recognizes MYH in rat ventricular extract
Date published: 2019-09-03
Rated 5 out of 5 by from Great Technical Service and Great Product I purchased this product one month ago and I must admit, I did have some bumps in the road when it came to receipt of the product and full understanding of the different variations. However, those bumps were easily smoothed out with a quick call to customer service and technical service helped me understand the product entirely. I'm very happy I ordered my antibodies from Santa Cruz Bio Technology.
Date published: 2017-07-07
Rated 2 out of 5 by from Weak for immunofluorescence I used this antibody in thick sections (200 um) of breast xenografts. The staining is not very specific and weak.
Date published: 2017-06-26
Rated 5 out of 5 by from Very good in WB in C2C12 cells Compare to MYH7 (A4.840), MYH (B5)shows strong signal in C2C12 differentiation for 7 days. It is a good antibody for WB in C2C12.
Date published: 2017-05-31
Rated 5 out of 5 by from Nice for WB The MYH(B-5) mouse monoclonal antibody is very nice for Western blotting!
Date published: 2017-05-24
Rated 5 out of 5 by from Very good antibody for WB Very good antibody for WB and excellent immunohistochemistry, I suggest dilution this antibody at 1:200.
Date published: 2017-02-09
Rated 5 out of 5 by from superior replacement MYH Antibody (B-5) is raised against the same recombinant protein immunogen used to develop the rabbit polyclonal sc-20641 (H-300) antibody. This new monoclonal antibody is a superior replacement with greater specificity and no lot-to-lot variation.
Date published: 2017-01-07
Rated 5 out of 5 by from Excellent results in Western Blot with human Excellent results in Western Blot with human, mouse and rat samples -SCBT QC
Date published: 2013-04-21
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