Anti-Muscle Actin Antibody (HUC1-1) recommended for detection of all vertebrate and avian Muscle Actins of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Muscle Actin Antibody (HUC1-1): sc-53141

Anti-Muscle Actin Antibody (HUC1-1) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-Muscle Actin Antibody (HUC1-1) is a mouse monoclonal IgG2a κ Muscle Actin antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against vascular smooth Actin of human origin
    • Anti-Muscle Actin Antibody (HUC1-1) is recommended for detection of all vertebrate and avian Muscle Actins of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with cytoplasmic Actins
    • Anti-Muscle Actin Antibody (HUC1-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Muscle Actin (HUC1-1): sc-53141.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Muscle Actin Antibody (HUC1-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Muscle Actin Antibody (HUC1-1) (see ordering information below).
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I am using Muscle Actin (HUC1-1): sc-53141 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works well in a wide variety of species for Works well in a wide variety of species for Western Blot. -SCBT QC
Date published: 2014-05-03
Rated 5 out of 5 by from Produces clean strong bands with no background Produces clean strong bands with no background in Westerns. -SCBT QC
Date published: 2013-11-09
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