Anti-Muscle Actin Antibody (HHF35) recommended for detection of muscle specific α and γ Actin isomers of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Muscle Actin Antibody (HHF35): sc-53014

Anti-Muscle Actin Antibody (HHF35) is rated 3.4 out of 5 by 5.
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Datasheets

    • Muscle Actin Antibody (HHF35) is a mouse monoclonal IgG1, cited in 3 publications, provided at 200 µg/ml
    • raised against myocardium of human origin
    • recommended for detection of muscle specific α and γ Actin isomers of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with β Actin isomers
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Muscle Actin Antibody (HHF35). This work is in progress.
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Are there any issues with using sc-53014: Muscle Actin (HHF35) mouse monoclonal primary antibody on mouse samples?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-53014 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from strong base band Immunoblot analysis of whole cell actin expression in HeLa gave a strong baseband display and was very clean.
Date published: 2017-01-10
Rated 1 out of 5 by from Très mauvais Ac pour les coupes en paraffine Très mauvais Ac pour les coupes en paraffine, pas de différence entre l'Ac et le contrôle négatif.
Date published: 2016-12-09
Rated 5 out of 5 by from Shows a strong Shows a strong, clear band with HeLa whole cell extract. -SCBT QC
Date published: 2015-01-06
Rated 4 out of 5 by from Successfully stained myocardial Successfully stained myocardial, skeletal muscle and smooth muscle cells. -SCBT QC
Date published: 2014-10-27
Rated 2 out of 5 by from Had difficulty with staining parrafin Had difficulty with staining parrafin-embedded human sections.
Date published: 2012-09-20
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