Anti-Muscle Actin Antibody (5G2) recommended for detection of muscle specific α and γ Actin isomers of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Muscle Actin Antibody (5G2): sc-71626

Anti-Muscle Actin Antibody (5G2) is rated 4.0 out of 5 by 1.
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Datasheets

    • Muscle Actin Antibody (5G2) is a mouse monoclonal IgG1, cited in 1 publications, provided at 200 µg/ml
    • raised against myocardium of human origin
    • recommended for detection of muscle specific α and γ Actin isomers of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with beta Actin isomers
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Muscle Actin Antibody (5G2). This work is in progress.
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Can sc-71626: Muscle Actin (5G2) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, sc-71626: Muscle Actin (5G2) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Western blot looks good with A Western blot looks good with A-10 whole cell lysate, 50 ug protein load. -SCBT QC
Date published: 2013-07-16
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