Anti-Muscle Actin Antibody (4i346) recommended for detection of muscle specific α and γ Actin isomers of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Muscle Actin Antibody (4i346): sc-71625

Anti-Muscle Actin Antibody (4i346) is rated 4.0 out of 5 by 2.
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Datasheets

    • Muscle Actin Antibody (4i346) is a mouse monoclonal IgG1, cited in 1 publications, provided at 200 µg/ml
    • raised against myocardium of human origin
    • recommended for detection of muscle specific α and γ Actin isomers of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with beta Actin isomers
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Muscle Actin Antibody (4i346). This work is in progress.
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What blocking solution should I use with Muscle Actin (4i346): sc-71625 monoclonal antibody for Western blot?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Can detect both endogenous and transfected Can detect both endogenous and transfected muscle actin. -SCBT QC
Date published: 2014-09-03
Rated 4 out of 5 by from Robust signal in HeLa whole cell lysate Western Robust signal in HeLa whole cell lysate Western Blot at 1:100. -SCBT QC
Date published: 2014-03-08
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