mucolipin 1 Double Nickase Plasmid (m): sc-430117-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mucolipin 1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- mucolipin 1 Double Nickase Plasmid (m) and mucolipin 1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mcoln1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mucolipin 1 Double Nickase Plasmid (m) | sc-430117-NIC | 20 µg | $410.00 | |||
mucolipin 1 Double Nickase Plasmid (m2) | sc-430117-NIC-2 | 20 µg | $410.00 |
Mcoln1 encodes mucolipin 1 (TRPML1), an endolysosomal Ca2+-permeable cation channel that regulates lysosomal ion homeostasis, membrane trafficking, and fusion–fission dynamics. By coupling luminal Ca2+ release to downstream effectors, TRPML1 supports autophagy–lysosome pathway flux, endocytic cargo processing, and lysosomal exocytosis important for cellular clearance and nutrient sensing. Mcoln1-dependent signaling intersects with lysosomal stress responses and vesicular transport programs that influence mitochondrial quality control and inflammatory signaling. Disruption of TRPML1 function is linked to lysosomal storage pathology and neurodegenerative phenotypes, making Mcoln1 a key target for mechanistic studies of endolysosomal dysfunction in mouse models.
mucolipin 1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Mcoln1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Mcoln1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Mcoln1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Mcoln1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.