MRP1 Antibody (QCRL-1)recommended for detection of an epitope between amino acids 918-924 of MRP1 of human origin by WB, IP, IF, IHC(P) and FCM

MRP1 Antibody (QCRL-1): sc-18835

MRP1 Antibody (QCRL-1) is rated 4.8 out of 5 by 19.
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Datasheets

    • MRP1 Antibody (QCRL-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a human small cell lung cancer cell line H69AR
    • MRP1 Antibody (QCRL-1) is recommended for detection of an epitope between amino acids 918-924 of MRP1 of human origin by WB, IP, IF, IHC(P) and FCM
    • MRP1 Antibody (QCRL-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MRP1 (QCRL-1): sc-18835.
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What experimental procedure should be used when using the conjugated MRP1 (QCRL-1, sc-18835) monoclonal antibody in flow cytometry? Intracellular or surface staining?

Asked by: K S K
Thank you for your question. We recommend using a protocol of direct staining of cell surface antigens (live cells) with this conjugated mouse monoclonal antibody, MRP1 (QCRL-1): sc-18835. A link to our recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-05-12

What negative control should be used when using the conjugated version of MRP1 (QCRL-1): sc-18835 monoclonal antibody in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-18835 PE, then you would want to use normal mouse IgG1 PE, sc-2866.
Answered by: Technical Support 10
Date published: 2017-01-10
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Rated 5 out of 5 by from Good! Good antibody work well in WB at 1:50 in vesicle preparation from insect cells infected with baculovirus containing human MRP1 cDNA (Corning® Gentest™ Human MRP1 Vesicles, Product #453455)
Date published: 2018-01-22
Rated 5 out of 5 by from Clean blot This antibody specifically identifies MRP1 by WB. You will be able to get clean blots. If it does not work for you, try to optimize your lysis method instead.
Date published: 2017-11-29
Rated 3 out of 5 by from IHC for TNBC I don't know why so MRP1 signal is week in triple negative breast cancer section. Maybe it expressed very low level of MRP1 in breast cancer tissue?
Date published: 2017-10-27
Rated 4 out of 5 by from Good Features I applied a sample of it a month ago. I used this antibody in Immunohistochemistry (dilution 1:75) and got good staining in human stomach tissue and lung cancer tissue. Antibody incubation time is 60min. I used EDTA for antigen retrieval.
Date published: 2017-10-20
Rated 5 out of 5 by from great great antibody work well in WB at 1:300, bladder cancer
Date published: 2017-07-06
Rated 5 out of 5 by from IHC And WB !! Pleased with both western blot and IHC staining data
Date published: 2017-01-13
Rated 5 out of 5 by from Excellent, versatile antibody The MRP1 antibody (QCRL-1) is a great, multi-functional antibody with over 20 citations for varying applications, including immunohistochemistry and Western blot.
Date published: 2016-12-20
Rated 5 out of 5 by from outstanding for MRP1 Working quite well ,The experimental result is satisfactory
Date published: 2016-11-16
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