MPP1 Antibody (H-7)recommended for detection of MPP1 of human origin by WB, IP, IF and ELISA

MPP1 Antibody (H-7): sc-515194

MPP1 Antibody (H-7) is rated 4.3 out of 5 by 3.
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Datasheets

    • MPP1 Antibody (H-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1557-1675 mapping near the C-terminus of MPP1 of human origin
    • MPP1 Antibody (H-7) is recommended for detection of MPP1 of human origin by WB, IP, IF and ELISA
    • MPP1 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MPP1 (H-7): sc-515194.
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What is the recommended starting dilution for Western blot for sc-515194: MPP1 (H-7) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from works well with human samples This antibody works well for Western blot in 293 cells, and the dilution for WB is 1:1000
Date published: 2017-07-25
Rated 4 out of 5 by from MPP1 antibody in various cancer cell lines Been working on characterizing the expression of KIF20B/MPP1 in cancer cell lines. had a tough time seeing expression until I loaded 150ug (which I know is a lot). ES-2 positive control is 50ug. Overnight incubation with primary antibody dilution 1:1000 in 5% milk. Secondary dilution, 1:5000. Immunofluorescence data (not shown) indicates high expression in nucleus.
Date published: 2017-04-26
Rated 4 out of 5 by from Very nice for Western blotting - IP is uncertain sc-515194 works for westerns but maybe not for IPs (the spec sheet says it works for IP). 1.5mg of lysate was used with 10ug of antibody. 5% input showed a signal but nothing was in the IP. The IP was done by an experienced researcher.
Date published: 2016-12-30
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