MPP1 Antibody (H-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 1557-1675 mapping near the C-terminus of MPP1 of human origin
MPP1 Antibody (H-7) is recommended for detection of MPP1 of human origin by WB, IP, IF and ELISA
MPP1 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MPP1 (H-7): sc-515194.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The Kinesins constitute a large family of microtubule-dependent motor proteins which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual Kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events, including endocytosis and transcytosis. MPP1 (M-phase phosphoprotein 1), also known as KIF20B (kinesin family member 20B), MPHOSPH1 or KRMP1, is a 1,820 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one kinesin-motor domain. Expressed in kidney, brain, testis and ovary, MPP1 functions as a plus-end directed motor enzyme that interacts with Pin1 and is required for the completion of cytokinesis. MPP1, which exists as multiple alternatively spliced isoforms termed 1-5, is subject to post-translational phosphorylation, probably by ATM or ATR.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-515194: MPP1 (H-7) monoclonal antibody?
Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
Rated 5 out of
works well with human samplesThis antibody works well for Western blot in 293 cells, and the dilution for WB is 1:1000
Date published: 2017-07-25
Rated 4 out of
MPP1 antibody in various cancer cell linesBeen working on characterizing the expression of KIF20B/MPP1 in cancer cell lines. had a tough time seeing expression until I loaded 150ug (which I know is a lot). ES-2 positive control is 50ug. Overnight incubation with primary antibody dilution 1:1000 in 5% milk. Secondary dilution, 1:5000. Immunofluorescence data (not shown) indicates high expression in nucleus.
Date published: 2017-04-26
Rated 4 out of
Professor Griffin from
Very nice for Western blotting - IP is uncertainsc-515194 works for westerns but maybe not for IPs (the spec sheet says it works for IP). 1.5mg of lysate was used with 10ug of antibody. 5% input showed a signal but nothing was in the IP. The IP was done by an experienced researcher.
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