mouse IgG lambda binding protein (m-IgGλ BP) conjugated to CruzFluor™ 680 (CFL 680)
supplied at 200 µg in 0.5 ml volume
CruzFluor™ 680 (CFL 680) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 680, offering comparable photostability and the ability to resist protein quenching
Excitation of 683 nm, Emission of 700 nm
Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF) and Flow Cytometry (FCM)
Suitable for binding to about 51%, but not all, mouse IgGλ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGκ light chain antibodies
Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-CFL 680 (sc-516180)
For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary antibody and mouse IgG binding protein incubations.
Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
If you have an interest in testing a 10 µg sample of m-IgGλ BP-CFL 680 (sc-516194) our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 680 (CFL 680) reagents, please contact our Technical Service Department (or your local Distributor) for more information.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US