m-IgG lambda BP-CFL 555 mouse IgG lambda binding protein (m-IgGλ BP) conjugated to Cruz Fluor™ 555, for WB (RGB), IF, IHC(P) and FCM

m-IgGλ BP-CFL 555: sc-516191

m-IgGλ BP-CFL 555 is rated 5.0 out of 5 by 1.
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Datasheets

    • mouse IgG lambda binding protein (m-IgGλ BP) conjugated to CruzFluor™ 555 (CFL 555)
    • CruzFluor™ 555 (CFL 555) is a orange/red fluorescent dye that is an excellent substitute for Alexa Fluor® 555, offering comparable photostability and the ability to resist protein quenching
    • supplied at 200 µg in 0.5 ml volume
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to about 51%, but not all, mouse IgGλ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGκ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-CFL 555 (sc-516177)
    • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
    • If you have an interest in testing a 10 µg sample of m-IgGλ BP-CFL 555 (sc-516191) our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 555 (CFL 555) reagents, please contact our Technical Service Department (or your local Distributor) for more information.
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Rated 5 out of 5 by from good antibody for IHC It worked with 1:500 dilution using brain sections, but the background is a bit strong, still a good antibody
Date published: 2017-04-05
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