m-IgG lambda BP-CFL 488 mouse IgG lambda binding protein (m-IgGλ BP) conjugated to Cruz Fluor™ 488, for WB (RGB), IF, IHC(P) and FCM

m-IgGλ BP-CFL 488: sc-516190

m-IgGλ BP-CFL 488 is rated 3.0 out of 5 by 4.
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Datasheets

    • mouse IgG lambda binding protein (m-IgGλ BP) conjugated to CruzFluor™ 488 (CFL 488)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 488 (CFL 488) is a green fluorescent dye that is an excellent substitute for Alexa Fluor® 488, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 488 nm, Emission of 514 nm
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to about 51%, but not all, mouse IgGλ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGκ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-CFL 488 (sc-516176)
    • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
    • If you have an interest in testing a 10 µg sample of m-IgGλ BP-CFL 488 (sc-516190), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 488 (CFL 488) reagents, please contact our Technical Service Department (or your local Distributor) for more information.
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Rated 1 out of 5 by from Did not work for immunofluorescence Did not work for immunofluorescence. Did not work on mouse cells or human cells.
Date published: 2019-05-01
Rated 4 out of 5 by from no objections so far I've recently tried m-IgG BP-CFL 488 antibody and I am satisfied with the result.
Date published: 2017-11-27
Rated 3 out of 5 by from Good Quite good antibody. The sc-516177 antibody gives better result in IF analysis. Dilution 1:100
Date published: 2017-11-17
Rated 4 out of 5 by from Ok result I tried both kappa and lambda CFL 488 binding proteins as I had both types of antibodies. Loved the specificity of the kappa binding protein. This lambda product gave decent results, but not as sharp as the kappa product.
Date published: 2016-11-30
  • y_2021, m_10, d_16, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
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  • clientName_scbt
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