m-IgG kappa BP-CFL 680 mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Cruz Fluor™ 680, for NIR WB, IF, IHC(P) and FCM

m-IgGκ BP-CFL 680: sc-516180

m-IgGκ BP-CFL 680 is rated 4.7 out of 5 by 19.
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Datasheets

    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 680 (CFL 680)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 680 (CFL 680) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 680, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 683 nm, Emission of 700 nm
    • Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to about 46%, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary antibody and mouse IgG binding protein incubations.
    • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
    • If you have an interest in testing a 10 µg sample of m-IgGκ BP-CFL 680 (sc-516180) or m-IgGκ BP-CFL 790 (sc-516181), please contact our Technical Service Department (or your local Distributor) for more information.
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I want to use this antibody.  First IP my sample with a mouse IgG1 monoclonal antibody, run a wb with Santa Cruz primary antibodies. Will this antibody react to the IgG of the antibody used for the IP? 

Asked by: science1387
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-01-28

will the SC-516181 secondary antibody recognize SC-377218? If not what near infrared 790 should I use?

Asked by: Scott1
Thank you for your question. Yes, you could use our m-IgGκ BP-CFL 680: sc-516180 as a secondary antibody for detection of Trk B Antibody (F-1): sc-377218 which is a mouse monoclonal IgM (kappa light chain). This secondary antibody, m-IgGκ BP-CFL 680: sc-516180, is suitable for detection of both mouse IgG and mouse IgM with kappa light chains.
Answered by: Technical Support
Date published: 2020-03-24

Does this secondary react with denatured mouse IgG light or heavy chains? Would like to use it in a Co-IP experiment. Thank you.

Asked by: zz1234
This mouse IgG binding protein (secondary) specifically binds to the kappa light chain of mouse IgG, whether it is denatured or not. It will not recognize mouse heavy chain IgG.
Answered by: Technical Support
Date published: 2019-01-06

Is this reagent mouse specific, ie how much cross reactivity would I expect with rat and mouse as primary antibodies.

Asked by: curosity
Thank you for your question. We do not expect this mouse IgG binding protein to have any cross-reactivity with primary antibodies raised in rat, and will cover this by warranty.
Answered by: Technical Service
Date published: 2017-02-09
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Rated 2 out of 5 by from Western blotting Non-specific bands were detected using this second antibody.
Date published: 2019-12-06
Rated 5 out of 5 by from great secondary for immunoblots works great with licor system. very clean background
Date published: 2019-07-30
Rated 4 out of 5 by from Works well Working good in both BSA and casein buffer system (1:1000, 200ug/0.5ml)
Date published: 2018-01-03
Rated 5 out of 5 by from Work good We bought a Santa beta-actin and a srebf1 antibody, they both worked well, so did this anti-antibody sc-516180.
Date published: 2017-09-30
Rated 5 out of 5 by from Works very well This antibody is working very well and I got it that I want.
Date published: 2017-08-23
Rated 4 out of 5 by from Works great Use a lower dilution to avoid too much background.
Date published: 2017-08-03
Rated 5 out of 5 by from Excellent We tested this antibody against LiCOR Donkey anti-mouse-IRdye 680. I think it worked better. There wa a much stronger single... will have to dilute.
Date published: 2017-07-28
Rated 5 out of 5 by from Good Nice specificity. Suitable for Licor. Works at low concentration also.
Date published: 2017-06-08
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