m-IgGκ BP-CFL 680: sc-516180
5
4
3
2
1
- mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 680 (CFL 680)
- supplied at 200 µg in 0.5 ml volume
- CruzFluor™ 680 (CFL 680) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 680, offering comparable photostability and the ability to resist protein quenching
- Excitation of 683 nm, Emission of 700 nm
- Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
- Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF) and Flow Cytometry (FCM)
- Suitable for binding to most, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
- Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
- For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary antibody and mouse IgG binding protein incubations.
- Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
QUICK LINKS
SEE ALSO...
- Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
I want to use this antibody. First IP my sample with a mouse IgG1 monoclonal antibody, run a wb with Santa Cruz primary antibodies. Will this antibody react to the IgG of the antibody used for the IP?
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Date published: 2021-01-28
will the SC-516181 secondary antibody recognize SC-377218? If not what near infrared 790 should I use?
Thank you for your question. Yes, you could use our m-IgGκ BP-CFL 680: sc-516180 as a secondary antibody for detection of Trk B Antibody (F-1): sc-377218 which is a mouse monoclonal IgM (kappa light chain). This secondary antibody, m-IgGκ BP-CFL 680: sc-516180, is suitable for detection of both mouse IgG and mouse IgM with kappa light chains.
Date published: 2020-03-24
Does this secondary react with denatured mouse IgG light or heavy chains? Would like to use it in a Co-IP experiment. Thank you.
This mouse IgG binding protein (secondary) specifically binds to the kappa light chain of mouse IgG, whether it is denatured or not. It will not recognize mouse heavy chain IgG.
Date published: 2019-01-06
Is this reagent mouse specific, ie how much cross reactivity would I expect with rat and mouse as primary antibodies.
Thank you for your question. We do not expect this mouse IgG binding protein to have any cross-reactivity with primary antibodies raised in rat, and will cover this by warranty.
Date published: 2017-02-09
Rated 5 out of
5
by
dudulff from
cheap but works well
It works fine with the bio-rad Chemi Doc MP imaging system. I would also try it as a substitute of anti-mouse IgM secondary antibody.
Date published: 2022-08-26
Rated 2 out of
5
by
western1 from
Western blotting
Non-specific bands were detected using this second antibody.
Date published: 2019-12-06
Rated 5 out of
5
by
aurora2019 from
great secondary for immunoblots
works great with licor system. very clean background
Date published: 2019-07-30
Rated 4 out of
5
by
SCJHU from
Works well
Working good in both BSA and casein buffer system (1:1000, 200ug/0.5ml)
Date published: 2018-01-03
Rated 5 out of
5
by
yipupa from
Work good
We bought a Santa beta-actin and a srebf1 antibody, they both worked well, so did this anti-antibody sc-516180.
Date published: 2017-09-30
Rated 5 out of
5
by
shinyh from
Works very well
This antibody is working very well and I got it that I want.
Date published: 2017-08-23
Rated 4 out of
5
by
ben1347 from
Works great
Use a lower dilution to avoid too much background.
Date published: 2017-08-03
Rated 5 out of
5
by
AP128 from
Excellent
We tested this antibody against LiCOR Donkey anti-mouse-IRdye 680. I think it worked better. There wa a much stronger single... will have to dilute.
Date published: 2017-07-28