m-IgG kappa BP-CFL 647 mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Cruz Fluor™ 647, for WB (RGB), IF, IHC(P) and FCM

m-IgGκ BP-CFL 647: sc-516179

m-IgGκ BP-CFL 647 is rated 5.0 out of 5 by 3.
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Datasheets

    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 647 (CFL 647)
    • CruzFluor™ 647 (CFL 647) conjugated secondaries are similar to Alexa Fluor® 647 and Cy5® fluorescent dyes, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 654 nm, Emission of 669 nm
    • supplied at 200 µg in 0.5 ml volume
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to about 46%, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
    • If you have an interest in testing a 10 µg sample of m-IgGκ BP-CFL 647 (sc-516179) our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 647 (CFL 647) reagents, please contact our Technical Service Department (or your local Distributor) for more information.
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Rated 5 out of 5 by from Great for intracellular staining-flow cytometry Stained HEK 293T (human) and B16F10 (mouse) cells using this antibody and ran flow cytometry. The staining was clear without much background and the human and mouse cells gave a good Stain Index. The target was Ku70 protein. I used the antibody at a 1:200 dilution.
Date published: 2018-06-18
Rated 5 out of 5 by from Great Highly specific and bright in HeLa cells for IF staining!
Date published: 2017-11-27
Rated 5 out of 5 by from Great experience! I called them to get sample for 2-3 antibodies and they shipped it for me right away. It was a great experience\!
Date published: 2017-08-08
  • y_2021, m_12, d_5, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
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  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_516179, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
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  • REVIEWS, PRODUCT