m-IgG kappa BP-CFL 555 mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Cruz Fluor™ 555, for WB (RGB), IF, IHC(P) and FCM

m-IgGκ BP-CFL 555: sc-516177

m-IgGκ BP-CFL 555 is rated 5.0 out of 5 by 16.
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Datasheets

    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 555 (CFL 555)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 555 (CFL 555) is a orange/red fluorescent dye that is an excellent substitute for Alexa Fluor® 555, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 556 nm, Emission of 569 nm
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to about 46%, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
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What is it for?

Asked by: O man
Thank you for your question. This product is a mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 555 (CFL 555). This mouse IgG binding protein is a strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for use in immunofluorescence (IF) and Flow Cytometry (FCM) research applications. It is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. CruzFluor™ 555 (CFL 555) is a orange/red fluorescent dye that is an excellent substitute for Alexa Fluor® 555, but CruzFluor™ dyes have a superior ability to resist protein quenching, and offer greater photostability. Please contact our Technical Service department if you would like further information about our new mouse IgG binding proteins. Please call us directly at (800) 457-3801 option 2, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-06
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Rated 5 out of 5 by from 东西不错 这产品适合我现在的需要 需要购买一些来使用,希望实验做下来可以顺利。期待后期可以继续使用贵公司的产品
Date published: 2018-05-14
Rated 5 out of 5 by from Good results in WB I was working with fluorescent WB and this 2ry ab worked perfectly!
Date published: 2018-02-01
Rated 5 out of 5 by from Brilliant Excellent on HEK293 and cryosections of chick embryos
Date published: 2018-01-03
Rated 5 out of 5 by from Works great Use this secondary ab for IF of cell samples, works great.
Date published: 2017-12-05
Rated 5 out of 5 by from Great This sample shows highly specific staining and great signal in HeLa cells.
Date published: 2017-11-27
Rated 5 out of 5 by from GREAT I use this antibody (1:100) in my Leukemia cells, it works very well.
Date published: 2017-11-20
Rated 5 out of 5 by from Clear signal under flourescent microscope This antibody was used at 1:100 and it worked very well to detect sc-59737 in HEK293 cells
Date published: 2017-09-11
Rated 5 out of 5 by from great antibody for immunohistochemistry, 1:500 worked well using tissue extracted from the brain.
Date published: 2017-07-04
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