m-IgG kappa BP-CFL 488mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Cruz Fluor™ 488, for WB (RGB), IF, IHC(P) and FCM

m-IgGκ BP-CFL 488: sc-516176

m-IgGκ BP-CFL 488 is rated 4.1 out of 5 by 26.
  • y_2019, m_12, d_6, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_26
  • loc_en_US, sid_516176, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 4ms
  • REVIEWS, PRODUCT
5
16
4
5
3
1
2
0
1
4

Datasheets

    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 488 (CFL 488)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 488 (CFL 488) is a green fluorescent dye that is an excellent substitute for Alexa Fluor® 488, but CruzFluor™, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 488 nm, Emission of 514 nm
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to mouse IgGκ light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
Submit a review for this product and receive 15 CruzCredits

Is it feasible to use this antibody for immunohistochemistry?

Asked by: Pramod
Thank you for your question. Yes, this anti-mouse IgG (kappa) secondary antibody can be used for fluorescent detection in tissues.
Answered by: Technical Support
Date published: 2019-08-17

unfortunately you do not give the relevant details of the nature of the protein but it appears to be a recombinant antibody fragment. Can you indicate the molecular weight and if it is free of myc or his tails or which one it might contain?

Asked by: Gerhard
Thank you for your question. Unfortunately this information is proprietary, but we recommend that you reach out to technical service to see if we can help you with the information you need in order to use this secondary antibody reagent effectively.
Answered by: Tech Service
Date published: 2019-03-20

I am interested in this antibody. However, I am curious to know the host in order to select a proper blocking serum

Asked by: Nada hussein
Thank you for your question. For IF and IHC blocking we recommend using our UltraCruz Blocking reagent. You can find information about it here: https://www.scbt.com/scbt/product/ultracruz-blocking-reagent If you are using it for WB, you can using 5% milk with TBS-T. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2019-03-19

Can I also use this antibody for ELISA detection in a plate reader? Best, Jasmin

Asked by: JEF88
Thank you for your question! Yes, this should be fine, as long as you can "read" the green color spectra, this should be fine! If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-05-26

What is the advantage of using Cruz Fluor 488 conjugated binding protein over an Alexa Fluor 488 conjugated antibody?

Asked by: lpk346
Thank you for your question. There are several advantages to using our mouse IgG binding protein. The product is designed to be highly specific and to bind to only a very specific epitope on the kappa light chain of mouse IgG. Therefore, non-specific binding and background is greatly reduced compared to conventional anti-mouse IgG secondary antibodies. Our mouse IgG binding proteins are not produced in a host animal, and therefore there is no lot-to-lot variation, as is associated with conventional secondary antibodies. And in the case of this specific Cruz Fluor 488 conjugated binding protein, CruzFluor™ 488 (CFL 488) is a green fluorescent dye that is an excellent substitute for Alexa Fluor® 488, but CruzFluor™ dyes have a superior ability to resist protein quenching, and offer greater photostability.
Answered by: Technical Support
Date published: 2019-07-10

Is this really better than using a regular secondary antibody?

Asked by: SCM4
Thank you for your question. We recommend using our exclusive new mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 488 (CFL 488) as an alternative to a regular secondary antibody because it only detects a specific epitope on the kappa light chain of mouse IgG. The results show strong signal with minimal background. In addition, because this is not raised in a host animal, there is no lot-to-lot variation as with a conventional goat or rabbit anti-mouse IgG secondary antibody.
Answered by: Technical Support
Date published: 2018-08-16
  • y_2019, m_12, d_7, h_22CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_6
  • loc_en_US, sid_516176, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 3ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from good secondary antibody I received this antibody as a sample. It works very well at a dilution of 1:250 in inmunofluoresnce (in fixed cells) and the results of this IgGk antibody are better than the IgG Alexa Fluor antibodies.
Date published: 2019-07-29
Rated 5 out of 5 by from m-IgGκ BP-CFL 488 - good for IF worked well with standard protocol at 1:500 dilution; togethere with YAP (63.7) scbt product
Date published: 2018-07-13
Rated 4 out of 5 by from Works great in flow cytometry! I tested this antibody at a 1:200 dilution with HEK (human) cells and B16F10 (mouse) cells to stain for the nuclear protein Ku70, and analyzed using flow cytometry. For the HEK cells I got excellent Stain Index. For the B16 cells, the background was a bit high, but the results were as expected.
Date published: 2018-06-18
Rated 5 out of 5 by from good secondary antibody I received this as a sample. It works very well at a dilution of 1:200 in IF (cryosections) and results are comparable to Alexa Fluor antibodies, but it is less expensive, so it is a good alternative to Alexa Fluor antibodies if you want to save $$ and still achieve good staining.
Date published: 2018-02-27
Rated 5 out of 5 by from Super good signal in FL WB Using a 1:5000 dilution, it showed a nice and clear band in fluorescent WB
Date published: 2018-02-01
Rated 4 out of 5 by from Works on tissue samples Try this secondary Ab for mouse liver tissue. it works ok
Date published: 2017-12-05
Rated 4 out of 5 by from good results We obtained good IF images with the sample in HeLa cells
Date published: 2017-11-27
Rated 1 out of 5 by from No signal I used it in the highest recommended concentration with a SC mouse monoclonal primary antibody and I didn't see any signal. With our usual secondary antibody from an other company in a 10x bigger dilution I saw a nice picture.
Date published: 2017-11-01
  • y_2019, m_12, d_6, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_26
  • loc_en_US, sid_516176, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 3ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.