mouse anti-rabbit IgG-CFL 790 mouse monoclonal secondary antibody conjugated to CruzFluor™ 790 (CFL 790), for NIR WB, IF, IHC(P) and FCM

mouse anti-rabbit IgG-CFL 790: sc-516253

mouse anti-rabbit IgG-CFL 790 Secondary Antibody is rated 5.0 out of 5 by 1.
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Datasheets

    • mouse monoclonal secondary antibody conjugated to CruzFluor™ 790 (CFL 790)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 790 (CFL 790) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 790, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 786 nm, Emission of 811 nm
    • Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
    • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
    • For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary and secondary antibody incubations.
    • If you have an interest in testing a 10 µg sample of mouse anti-rabbit IgG-CFL 790 (sc-516253), our highly recommended monoclonal secondary antibody alternative to polyclonal anti-rabbit IgG-CFL 790 secondary antibodies, please contact our Technical Service Department (or your local Distributor) for more information.
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Would this AB work for immunofluorescence staining of cells?

Asked by: Patrycja
Thank you for your question. This secondary antibody is conjugated to CruzFluor™ 790 (CFL 790), which is a near infrared fluorescent dye. It could be used for immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
Answered by: Technical Support
Date published: 2019-05-25

做WESTERN BLOT,STAT3和EDG1都是兔来源一抗,扫膜用的是Odyssey的红外荧光成像,是否都可用sc-516253这款二抗?

Asked by: chenang
感谢您的提问。sc-516253 suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems,和一抗无关的。关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. sc-516253 suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-05-02
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Rated 5 out of 5 by from Great band I received this antibody recently. I used it for western blot and I got a nice band.
Date published: 2017-08-31
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