mouse anti-rabbit IgG-CFL 680 mouse monoclonal secondary antibody conjugated to CruzFluor™ 680 (CFL 680), for NIR WB, IF, IHC(P) and FCM

mouse anti-rabbit IgG-CFL 680: sc-516252

mouse anti-rabbit IgG-CFL 680 Secondary Antibody is rated 4.4 out of 5 by 5.
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Datasheets

    • mouse monoclonal secondary antibody conjugated to CruzFluor™ 680 (CFL 680)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 680 (CFL 680) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 680, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 683 nm, Emission of 700 nm
    • Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
    • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
    • For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary and secondary antibody incubations.
    • If you have an interest in testing a 10 µg sample of mouse anti-rabbit IgG-CFL 680 (sc-516252), our highly recommended monoclonal secondary antibody alternative to polyclonal anti-rabbit IgG-CFL 680 secondary antibodies, please contact our Technical Service Department (or your local Distributor) for more information.
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Rated 2 out of 5 by from Western blotting Non-specific bands were detected using this second antibody.
Date published: 2019-12-06
Rated 5 out of 5 by from western blot using anti-rabbit IgG-CFL This secondary antibody worked brilliantly for western blot and gave a strong band on the LiCor system at a dilution of 1:25,000
Date published: 2018-04-17
Rated 5 out of 5 by from Very good staining! This antibody produced very good results at a 1:200 dilution for staining mouse calvariae for immunofluorescence imaging.
Date published: 2018-03-16
Rated 5 out of 5 by from Nice band! I got the nice band and I am satisfying for this antibody.
Date published: 2017-08-23
Rated 5 out of 5 by from Good, Suitable for me. I am satisfied with the antibody as per the price concern. It works very well. Also work at 1:10000 dilution
Date published: 2017-06-08
  • y_2021, m_10, d_16, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_en_US, sid_516252, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
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  • REVIEWS, PRODUCT