MOPS [3-(N-Morpholino)propanesulfonic Acid] is a morpholino propanesulfonic acid, and structural analog to MES (first introduced by Good et al.) Both series of buffers were developed to meet the following criteria: midrange pKa, maximum water solubility, minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature chemically and enzymatically stable, minimal absorption in visible or UV spectral range, and reasonably is easily synthesized. pKa=7.2 at 25°C, Effective buffering range: pH 6.5-8.0.
1. Ellis, K.J. and Morrison, J.F. 1982. Methods Enzymol. 87: 405-426. PMID: 7176924 2. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950 3. Tucker, D.L., et al. 2002. J. Bacteriol. 184: 6551-6558. PMID: 12426343 4. Petrou, M.A. and Shanson, D.C. 2000. J. Antimicrob. Chemother. 46: 815-818. PMID: 11062205
• This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
Soluble in water (523 g/l at 20° C).
Store at room temperature
1.41 g/cm3 at 20° C
n20D ~1.51 (Predicted)
pKa: 7.2 (25° C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Akram AC; et alAkram AC; et al. (PubMed ID: 25555225) determined that MOPS, Sodium is a buffer that causes the least spontaneous fluorescein diacetate breakdown without inhibiting enzymatic activity. -SCBT Publication Review
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