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MMP9 Antibody (E-11) | SCBT - Santa Cruz Biotechnology
MMP9 Antibody (E-11) recommended for detection of MMP-9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

MMP9 Antibody (E-11): sc-393859

MMP-9 Antibody (E-11) is rated 4.5 out of 5 by 37.
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Datasheets

    • MMP9 Antibody (E-11) is a mouse monoclonal IgG2b, cited in 133 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 678-707 at the C-terminus of MMP-9 of human origin
    • Anti-MMP9 Antibody (E-11) is recommended for detection of MMP-9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-MMP9 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MMP9 (E-11): sc-393859.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for MMP9 Antibody (E-11) for WB and IHC(P) applications. These reagents are now offered in bundles with MMP9 Antibody (E-11) (see ordering information below).
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The MMP-9 band was observed at a size lower than that of MMP-1 (SC-58377). Is the size on the data sheet correct?

Asked by: ssy33144
Thank you for your question. Please contact our technical service directly so we can assist you with your experiment.
Answered by: Tech Support Europe
Date published: 2020-06-18

Hi, I have purchased this MMP9 monoclone antibody. I extracted total protein from HTR8 cell and loaded 50ug protein for western blot. Although I have tried for 3times, No signal was detected on the PVDF membrane. Could anyone help me with that please

Asked by: YipingYi
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

Which secondary antibody should I use with this for Westerns?

Asked by: Anna99
Try our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 4 out of 5 by from Good It is good for Western blot analysis. Dilution user 1:1000
Date published: 2022-04-07
Rated 4 out of 5 by from バンドが検出されます。 WBで使用しました。非特異的なバンドが検出されますが問題ないと思います。目的のバンドはよりシャープなバンドとして検出されました。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 3 out of 5 by from Western Blot I think the Band was found in 68 kD.... Could this happen?
Date published: 2019-01-09
Rated 4 out of 5 by from Good Ab for IHC staining of human MMP9 in FPPE Good Ab for IHC staining of MMP9 in FPPE samples of human tissue. Clean and specific staining
Date published: 2018-11-23
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from UPENN Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from useful in WB This antibody works good in western blot. I have applied it in many cell lines, such as MDA-MB-231, HEK293, and also mouse breast cancer cell 4T1, and a clear specific band was found in 92 kD.
Date published: 2017-11-04
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