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MMP9 Antibody (2C3) | SCBT - Santa Cruz Biotechnology
MMP9 Antibody (2C3) recommended for detection of MMP-9 of human origin by WB, IP, IF and IHC(P)

MMP9 Antibody (2C3): sc-21733

MMP-9 Antibody (2C3) is rated 4.7 out of 5 by 20.
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Datasheets

    • MMP9 Antibody (2C3) is a mouse monoclonal IgG1 κ, cited in 305 publications, provided at 200 µg/ml
    • raised against amino acids 603-614 of MMP-9 of human origin
    • MMP9 Antibody (2C3) is recommended for detection of MMP-9 of human origin by WB, IP, IF and IHC(P)
    • MMP9 Antibody (2C3) is available conjugated to agarose for IP
    • also available conjugated to Alexa Fluor® 594 for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See MMP9 (E-11): sc-393859 for MMP9 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MMP9 (2C3): sc-21733.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MMP9 Antibody (2C3) for WB and IHC(P) applications. These reagents are now offered in bundles with MMP9 Antibody (2C3) (see ordering information below).
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Have there been reports of a cleaved form of this metalloproteinase? I'm getting a single clear concise band near 45 kDa in different cells lines.

Asked by: AnaL
Thank you for your question. We are not aware of any splicing variants that would appear at that molecular weight. Please reach out to our technical service so we can look into this matter.
Answered by: Tech Support Europe
Date published: 2020-11-16

Is any of MMP9 antibodies working with horses

Asked by: helsheikh
Thank you for your question. This antibody has not yet been tested in horse samples so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-11-06

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-21
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Rated 4 out of 5 by from Good for Immunofluorescence The antibody works very well in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA.
Date published: 2020-11-10
Rated 3 out of 5 by from みえなかった。 サンプルでいただきました。癌細胞を使ってウエスタンブロットで確認。濃度は濃く使用しましたが、検出されませんでした。
Date published: 2020-08-19
Rated 5 out of 5 by from Great for immunofluorescence I purchased this Ab about a month ago and have had great results in my immunofluorescence staining.
Date published: 2019-11-06
Rated 1 out of 5 by from very faint band at 1:250 dilution I received the antibody to test on PMA-stimulated THP-1 cells, the 90kDa band is faint and there is unspecific band approx. 45kDa
Date published: 2019-04-25
Rated 5 out of 5 by from 目标条带清晰 在肠癌和乳腺癌的wb中,这支抗体有清晰的目标条带,未出现杂带,背景清晰,适合在wb中使用,稀释比例1:500
Date published: 2018-04-02
Rated 5 out of 5 by from Excellent product I got a free sample of this product last month and it work very well for my samples: cell line and tumor from mice
Date published: 2018-02-08
Rated 5 out of 5 by from western blot A clear and strong signal was showed in the lysis of HepG2 cells (1:400).
Date published: 2018-01-25
Rated 5 out of 5 by from GOOD Application Western blot Sample Rat Tissue lysate - whole (Colon) Gel Running Conditions Non-reduced Denaturing Loading amount 25 µg Specification Colon Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Date published: 2017-08-09
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